PMID- 21048306 OWN - NLM STAT- MEDLINE DCOM- 20110629 LR - 20231213 IS - 1347-5215 (Electronic) IS - 0918-6158 (Linking) VI - 33 IP - 11 DP - 2010 TI - miR-199a regulates the tumor suppressor mitogen-activated protein kinase kinase kinase 11 in gastric cancer. PG - 1822-7 AB - Small noncoding microRNAs (miRNAs) have been shown to play an important role in tumor proliferation and metastasis. However, their function and mechanism in the proliferation and metastasis of gastric cancer has not yet been elucidated. Here, we investigated the relationship between miRNA-199a and gastric cancer proliferation and metastasis. Using real-time reverse-transcriptase (RT)-polymerase chain reaction, we found that miR-199a is highly expressed in gastric cancer compared to normal gastric tissues and in metastatic, compared to non-metastatic gastric cancer tissues. MiR-199a positively regulated gastric cancer cell proliferation, migration and invasion. Further studies showed that mitogen-activated protein kinase kinase kinase 11 was significantly down-regulated by miR-199a at the post-transcriptional level and, the level of miR-199a expression in gastric cancer significantly correlated with clinical progression. These findings suggested miR-199a promoted proliferation and metastasis of gastric cancer cells through a regulatory pathway in gastric cancer that has yet to be described. miR-199a may be useful as a new potential therapeutic target for gastric cancer. FAU - Song, Guang AU - Song G AD - The First Affiliated Hospital of Harbin Medical University, Harbin 150001, China. FAU - Zeng, Huazong AU - Zeng H FAU - Li, Jian AU - Li J FAU - Xiao, Lifeng AU - Xiao L FAU - He, Yingzi AU - He Y FAU - Tang, Yinhua AU - Tang Y FAU - Li, Yuru AU - Li Y LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - Japan TA - Biol Pharm Bull JT - Biological & pharmaceutical bulletin JID - 9311984 RN - 0 (MicroRNAs) RN - EC 2.7.11.25 (MAP Kinase Kinase Kinases) SB - IM MH - Cell Line, Tumor MH - *Cell Proliferation MH - Down-Regulation MH - Gastric Mucosa/*metabolism MH - *Gene Expression Regulation, Neoplastic MH - Humans MH - MAP Kinase Kinase Kinases/*metabolism MH - MicroRNAs/*metabolism MH - Neoplasm Invasiveness/genetics/*physiopathology MH - Reverse Transcriptase Polymerase Chain Reaction MH - Stomach Neoplasms/genetics/*metabolism MH - Mitogen-Activated Protein Kinase Kinase Kinase 11 EDAT- 2010/11/05 06:00 MHDA- 2011/06/30 06:00 CRDT- 2010/11/05 06:00 PHST- 2010/11/05 06:00 [entrez] PHST- 2010/11/05 06:00 [pubmed] PHST- 2011/06/30 06:00 [medline] AID - JST.JSTAGE/bpb/33.1822 [pii] AID - 10.1248/bpb.33.1822 [doi] PST - ppublish SO - Biol Pharm Bull. 2010;33(11):1822-7. doi: 10.1248/bpb.33.1822.