PMID- 21050865
OWN - NLM
STAT- MEDLINE
DCOM- 20110121
LR  - 20211020
IS  - 1879-0631 (Electronic)
IS  - 0024-3205 (Print)
IS  - 0024-3205 (Linking)
VI  - 88
IP  - 1-2
DP  - 2011 Jan 3
TI  - Late phase ischemic preconditioning preserves mitochondrial oxygen metabolism and 
      attenuates post-ischemic myocardial tissue hyperoxygenation.
PG  - 57-64
LID - 10.1016/j.lfs.2010.10.022 [doi]
AB  - AIMS: Late phase ischemic preconditioning (LPC) protects the heart against 
      ischemia-reperfusion (I/R) injury. However, its effect on myocardial tissue 
      oxygenation and related mechanism(s) is unknown. The aim of the current study is 
      to determine whether LPC attenuates post-ischemic myocardial tissue 
      hyperoxygenation through preserving mitochondrial oxygen metabolism. MAIN 
      METHODS: C57BL/6 mice were subjected to 30 min coronary ligation followed by 60 
      min or 24 h reperfusion with or without LPC (3 cycles of 5 min I/5 min R): Sham, 
      LPC, I/R, and LPC+I/R group. Myocardial tissue Po(2) and redox status were 
      measured with electron paramagnetic resonance (EPR) spectroscopy. KEY FINDINGS: 
      Upon reperfusion, tissue Po(2) rose significantly above the pre-ischemic level in 
      the I/R mice (23.1 +/- 2.2 vs. 12.6 +/- 1.3 mmHg, p<0.01). This hyperoxygenation was 
      attenuated by LPC in the LPC+I/R mice (11.9 +/- 2.0 mmHg, p<0.01). Activities of 
      NADH dehydrogenase (NADH-DH), succinate-cytochrome c reductase (SCR) and 
      cytochrome c oxidase (CcO) were preserved or increased in the LPC group, 
      significantly reduced in the I/R group, and conserved in the LPC+I/R group. 
      Manganese superoxide dismutase (Mn-SOD) protein expression was increased by LPC 
      in the LPC and LPC+I/R mice compared to that in the Sham control (1.24 +/- 0.01 and 
      1.23 +/- 0.01, p<0.05). Tissue redox status was shifted to the oxidizing state with 
      I/R (0.0268 +/- 0.0016/min) and was corrected by LPC in the LPC+I/R mice (0.0379 +/- 
      0.0023/min). Finally, LPC reduced the infarct size in the LPC+I/R mice (10.5 +/- 
      0.4% vs. 33.3 +/- 0.6%, p<0.05). SIGNIFICANCE: Thus, LPC preserved mitochondrial 
      oxygen metabolism, attenuated post-ischemic myocardial tissue hyperoxygenation, 
      and reduced I/R injury.
CI  - Copyright (c) 2010 Elsevier Inc. All rights reserved.
FAU - Li, Yuanjing
AU  - Li Y
AD  - The Center for Biomedical EPR Spectroscopy and Imaging, Davis Heart and Lung 
      Research Institute and Division of Cardiovascular Medicine, Department of 
      Internal Medicine, The Ohio State University College of Medicine, Columbus, OH 
      43210, USA.
FAU - Cai, Ming
AU  - Cai M
FAU - Xu, Yi
AU  - Xu Y
FAU - Swartz, Harold M
AU  - Swartz HM
FAU - He, Guanglong
AU  - He G
LA  - eng
GR  - R01 HL081630-04/HL/NHLBI NIH HHS/United States
GR  - HL081630/HL/NHLBI NIH HHS/United States
GR  - R01 HL081630/HL/NHLBI NIH HHS/United States
GR  - R01 HL081630-02/HL/NHLBI NIH HHS/United States
GR  - R01 HL081630-03/HL/NHLBI NIH HHS/United States
GR  - R01 HL081630-04S1/HL/NHLBI NIH HHS/United States
GR  - P01 EB002180/EB/NIBIB NIH HHS/United States
GR  - R01 HL081630-01/HL/NHLBI NIH HHS/United States
GR  - P01EB2180/EB/NIBIB NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20101102
PL  - Netherlands
TA  - Life Sci
JT  - Life sciences
JID - 0375521
RN  - EC 1.- (Succinate Cytochrome c Oxidoreductase)
RN  - EC 1.6.99.3 (NADH Dehydrogenase)
RN  - EC 1.9.3.1 (Electron Transport Complex IV)
RN  - S88TT14065 (Oxygen)
SB  - IM
MH  - Animals
MH  - Blotting, Western
MH  - Electron Spin Resonance Spectroscopy
MH  - Electron Transport Complex IV/metabolism
MH  - *Ischemic Preconditioning, Myocardial
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mitochondria, Heart/*metabolism/physiology
MH  - Myocardial Infarction/enzymology/metabolism/physiopathology
MH  - Myocardial Reperfusion Injury/metabolism/physiopathology/*prevention & control
MH  - Myocardium/enzymology/metabolism
MH  - NADH Dehydrogenase/metabolism
MH  - Oxidation-Reduction
MH  - Oxygen/metabolism
MH  - Regional Blood Flow/physiology
MH  - Succinate Cytochrome c Oxidoreductase/metabolism
PMC - PMC3026613
MID - NIHMS250506
EDAT- 2010/11/06 06:00
MHDA- 2011/01/22 06:00
PMCR- 2012/01/03
CRDT- 2010/11/06 06:00
PHST- 2010/07/27 00:00 [received]
PHST- 2010/10/01 00:00 [revised]
PHST- 2010/10/19 00:00 [accepted]
PHST- 2010/11/06 06:00 [entrez]
PHST- 2010/11/06 06:00 [pubmed]
PHST- 2011/01/22 06:00 [medline]
PHST- 2012/01/03 00:00 [pmc-release]
AID - S0024-3205(10)00483-2 [pii]
AID - 10.1016/j.lfs.2010.10.022 [doi]
PST - ppublish
SO  - Life Sci. 2011 Jan 3;88(1-2):57-64. doi: 10.1016/j.lfs.2010.10.022. Epub 2010 Nov 
      2.