PMID- 21050897
OWN - NLM
STAT- MEDLINE
DCOM- 20110415
LR  - 20131121
IS  - 1878-1519 (Electronic)
IS  - 1569-9048 (Linking)
VI  - 175
IP  - 1
DP  - 2011 Jan 31
TI  - Effects of bone marrow-derived mononuclear cells on airway and lung parenchyma 
      remodeling in a murine model of chronic allergic inflammation.
PG  - 153-63
LID - 10.1016/j.resp.2010.10.006 [doi]
AB  - We hypothesized that bone marrow-derived mononuclear cells (BMDMC) would 
      attenuate the remodeling process in a chronic allergic inflammation model. 
      C57BL/6 mice were assigned to two groups. In OVA, mice were sensitized and 
      repeatedly challenged with ovalbumin. Control mice (C) received saline under the 
      same protocol. C and OVA were further randomized to receive BMDMC (2 x 10(6)) or 
      saline intravenously 24 h before the first challenge. BMDMC therapy reduced 
      eosinophil infiltration, smooth muscle-specific actin expression, subepithelial 
      fibrosis, and myocyte hypertrophy and hyperplasia, thus causing a decrease in 
      airway hyperresponsiveness and lung mechanical parameters. BMDMC from green 
      fluorescent protein (GFP)-transgenic mice transplanted into GFP-negative mice 
      yielded lower engraftment in OVA. BMDMC increased insulin-like growth factor 
      expression, but reduced interleukin-5, transforming growth factor-beta, 
      platelet-derived growth factor, and vascular endothelial growth factor mRNA 
      expression. In conclusion, in the present chronic allergic inflammation model, 
      BMDMC therapy was an effective pre-treatment protocol that potentiated airway 
      epithelial cell repair and prevented inflammatory and remodeling processes.
CI  - Copyright (c) 2010 Elsevier B.V. All rights reserved.
FAU - Abreu, Soraia C
AU  - Abreu SC
AD  - Laboratory of Pulmonary Investigation, Carlos Chagas Filho Institute of 
      Biophysics, Federal University of Rio de Janeiro, Rio de Janeiro, RJ, Brazil.
FAU - Antunes, Mariana A
AU  - Antunes MA
FAU - Maron-Gutierrez, Tatiana
AU  - Maron-Gutierrez T
FAU - Cruz, Fernanda F
AU  - Cruz FF
FAU - Carmo, Luana G R R
AU  - Carmo LG
FAU - Ornellas, Debora S
AU  - Ornellas DS
FAU - Junior, Humberto Carreira
AU  - Junior HC
FAU - Absaber, Alexandre M
AU  - Absaber AM
FAU - Parra, Edwin R
AU  - Parra ER
FAU - Capelozzi, Vera L
AU  - Capelozzi VL
FAU - Morales, Marcelo M
AU  - Morales MM
FAU - Rocco, Patricia R M
AU  - Rocco PR
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20101102
PL  - Netherlands
TA  - Respir Physiol Neurobiol
JT  - Respiratory physiology & neurobiology
JID - 101140022
RN  - 0 (Intercellular Signaling Peptides and Proteins)
RN  - 0 (Interleukin-5)
RN  - 9006-59-1 (Ovalbumin)
SB  - IM
MH  - Airway Remodeling/*physiology
MH  - Analysis of Variance
MH  - Animals
MH  - Bone Marrow Cells/*physiology
MH  - Bronchoalveolar Lavage Fluid
MH  - Cell- and Tissue-Based Therapy/*methods
MH  - Chronic Disease
MH  - Connective Tissue/*physiology/ultrastructure
MH  - Disease Models, Animal
MH  - Female
MH  - Injections, Intravenous/methods
MH  - Intercellular Signaling Peptides and Proteins/metabolism
MH  - Interleukin-5/metabolism
MH  - Leukocytes, Mononuclear/*physiology
MH  - Lung/pathology/ultrastructure
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Microscopy, Electron, Transmission/methods
MH  - Ovalbumin/immunology
MH  - Respiratory Hypersensitivity/etiology/pathology/*therapy
EDAT- 2010/11/06 06:00
MHDA- 2011/04/19 06:00
CRDT- 2010/11/06 06:00
PHST- 2010/09/07 00:00 [received]
PHST- 2010/10/17 00:00 [revised]
PHST- 2010/10/22 00:00 [accepted]
PHST- 2010/11/06 06:00 [entrez]
PHST- 2010/11/06 06:00 [pubmed]
PHST- 2011/04/19 06:00 [medline]
AID - S1569-9048(10)00395-2 [pii]
AID - 10.1016/j.resp.2010.10.006 [doi]
PST - ppublish
SO  - Respir Physiol Neurobiol. 2011 Jan 31;175(1):153-63. doi: 
      10.1016/j.resp.2010.10.006. Epub 2010 Nov 2.