PMID- 21051144
OWN - NLM
STAT- MEDLINE
DCOM- 20110328
LR  - 20190107
IS  - 1873-3336 (Electronic)
IS  - 0304-3894 (Linking)
VI  - 185
IP  - 2-3
DP  - 2011 Jan 30
TI  - Kinetics of cell lysis for Microcystis aeruginosa and Nitzschia palea in the 
      exposure to beta-cyclocitral.
PG  - 1214-20
LID - 10.1016/j.jhazmat.2010.10.033 [doi]
AB  - The effect of an algal metabolite, beta-cyclocitral, on the cell integrity of two 
      cyanobacteria and one diatom was investigated. The cyanobacteria, Microcystis 
      aeruginosa PCC 7005 and PCC 7820, and the diatom, Nitzschia palea, were exposed 
      to various concentrations of beta-cyclocitral. Scanning electron microscope (SEM) 
      results indicate that the cells of tested species were greatly altered after 
      being exposed to beta-cyclocitral. A flow cytometer coupled with the SYTOX stain and 
      chlorophyll-a auto-fluorescence was used to quantify the effect of beta-cyclocitral 
      on cell integrity for the tested cyanobacteria and diatom. Kinetic experiments 
      show that about 5-10 mg L(-1) of beta-cyclocitral for the two M. aeruginosa strains 
      and a much lower concentration, 0.1-0.5 mg L(-1), for N. palea were needed to 
      cause 15-20% of cells to rupture. When the beta-cyclocitral concentration was 
      increased to 200-1000 mg L(-1) for M. aeruginosa and 5-10 mg L(-1) for N. palea, 
      almost all the cells ruptured between 8 and 24h. A first-order kinetic model is 
      able to describe the data of cell integrity over time. The extracted rate 
      constant values well correlate with the applied beta-cyclocitral dosages. The 
      obtained kinetic parameters may be used to estimate beta-cyclocitral dosage and 
      contact time required for the control of cyanobacteria and diatoms in water 
      bodies.
CI  - Copyright (c) 2010 Elsevier B.V. All rights reserved.
FAU - Chang, De-Wei
AU  - Chang DW
AD  - Department of Environmental Engineering, National Cheng Kung University, Tainan 
      City 70101, Taiwan.
FAU - Hsieh, Meng-Ling
AU  - Hsieh ML
FAU - Chen, Yan-Min
AU  - Chen YM
FAU - Lin, Tsair-Fuh
AU  - Lin TF
FAU - Chang, Jo-Shu
AU  - Chang JS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20101015
PL  - Netherlands
TA  - J Hazard Mater
JT  - Journal of hazardous materials
JID - 9422688
RN  - 0 (Aldehydes)
RN  - 0 (Diterpenes)
RN  - 77Y0U2X29G (beta-cyclocitral)
SB  - IM
MH  - Aldehydes/*toxicity
MH  - Diatoms/cytology/*drug effects/ultrastructure
MH  - Diterpenes/*toxicity
MH  - Kinetics
MH  - Microcystis/cytology/*drug effects/ultrastructure
MH  - Models, Theoretical
EDAT- 2010/11/06 06:00
MHDA- 2011/03/29 06:00
CRDT- 2010/11/06 06:00
PHST- 2010/06/07 00:00 [received]
PHST- 2010/10/08 00:00 [revised]
PHST- 2010/10/09 00:00 [accepted]
PHST- 2010/11/06 06:00 [entrez]
PHST- 2010/11/06 06:00 [pubmed]
PHST- 2011/03/29 06:00 [medline]
AID - S0304-3894(10)01321-X [pii]
AID - 10.1016/j.jhazmat.2010.10.033 [doi]
PST - ppublish
SO  - J Hazard Mater. 2011 Jan 30;185(2-3):1214-20. doi: 10.1016/j.jhazmat.2010.10.033. 
      Epub 2010 Oct 15.