PMID- 21055231
OWN - NLM
STAT- MEDLINE
DCOM- 20110505
LR  - 20191210
IS  - 0529-5815 (Print)
IS  - 0529-5815 (Linking)
VI  - 48
IP  - 12
DP  - 2010 Jun 15
TI  - [The application of fluorescence in situ hybridization and automated image 
      cytometry in the diagnosis of urothelial carcinoma of bladder].
PG  - 933-6
AB  - OBJECTIVE: To discuss the application of automated DNA image cytometry (ICM) and 
      fluorescence in situ hybridization (FISH) in the diagnosis of urothelial 
      carcinoma of bladder. METHODS: From August 2008 to March 2009, 60 volunteers with 
      informed consent were divided into two groups, 40 patients proven as urothelial 
      carcinoma of bladder by pathology and 20 healthy individuals as control. Urine 
      was collected and tested by cytology, ICM and FISH. RESULTS: Overall sensitivity 
      of FISH was significantly higher in detection of malignancy than that of ICM 
      (82.5% vs 62.5%, P < 0.05) and that of urine cytology (82.5% vs 25.0%, P < 0.05), 
      while ICM was more sensitive to diagnose urothelial carcinoma of bladder than 
      urine cytology (62.5% vs 25.0%, P < 0.05). Specificities of urine cytology, ICM 
      and FISH were 100% in diagnosis of urothelial carcinoma of bladder (P > 0.05). 
      Sensitivities of urine cytology, ICM and FISH have no correlation with 
      pathological stage (P > 0.05), but have significant correlation with grade (P < 
      0.05). CONCLUSIONS: ICM and FISH have the same specificity as urine cytology in 
      diagnosis of urothelial carcinoma of bladder, but they have significantly higher 
      sensitivity than urine cytology. FISH has the highest sensitivity among three 
      diagnostic methods. Therefore, FISH may become a newly non-invasive technique for 
      the diagnosis and surveillance of urothelial carcinoma of bladder.
FAU - Gu, Shen-yang
AU  - Gu SY
AD  - Department of Urology, Clinical Medical College of Yangzhou University, Yangzhou 
      225001, China. maolujiu@yahoo.com
FAU - Yong, Yong-song
AU  - Yong YS
FAU - Wang, Ye-hua
AU  - Wang YH
FAU - Du, Yong-jun
AU  - Du YJ
LA  - chi
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhonghua Wai Ke Za Zhi
JT  - Zhonghua wai ke za zhi [Chinese journal of surgery]
JID - 0153611
SB  - IM
MH  - Aged
MH  - Aged, 80 and over
MH  - Case-Control Studies
MH  - Female
MH  - Humans
MH  - Image Cytometry/*methods
MH  - *In Situ Hybridization, Fluorescence
MH  - Male
MH  - Middle Aged
MH  - Sensitivity and Specificity
MH  - Urinary Bladder/pathology
MH  - Urinary Bladder Neoplasms/*diagnosis/pathology
EDAT- 2010/11/09 06:00
MHDA- 2011/05/06 06:00
CRDT- 2010/11/09 06:00
PHST- 2010/11/09 06:00 [entrez]
PHST- 2010/11/09 06:00 [pubmed]
PHST- 2011/05/06 06:00 [medline]
PST - ppublish
SO  - Zhonghua Wai Ke Za Zhi. 2010 Jun 15;48(12):933-6.