PMID- 21055805
OWN - NLM
STAT- MEDLINE
DCOM- 20110224
LR  - 20211020
IS  - 1878-5905 (Electronic)
IS  - 0142-9612 (Print)
IS  - 0142-9612 (Linking)
VI  - 32
IP  - 1
DP  - 2011 Jan
TI  - The surface molecular functionality of decellularized extracellular matrices.
PG  - 137-43
LID - 10.1016/j.biomaterials.2010.09.007 [doi]
AB  - Decellularization of tissues and organs is a successful platform technology for 
      creating scaffolding materials for tissue engineering and regenerative medicine. 
      It has been suggested that the success of these materials upon implantation is 
      due to the molecular signals provided by the remaining scaffold extracellular 
      matrix (ECM) components presented to probing cells in vivo as they repopulate the 
      surface. For this study, decellularized matrices were created from esophagus, 
      bladder, and small intestine harvested from adult male Fischer 344 rats. The 
      three decellularized matrices (each originating from source tissues which 
      included an epithelial lining on their luminal surfaces) were immunostained for 
      collagen IV and laminin to determine basement membrane retention. Scanning 
      electron micrographs of the surfaces were used to provide insight into the 
      surface topography of each of the decellularized tissues. Time-of-flight 
      secondary ion mass spectrometry (ToF-SIMS) was used to generate high-resolution 
      mass spectra for the surfaces of each scaffold. This surface-sensitive technique 
      allows for detailed molecular analysis of the outermost 1-2 nm of a material and 
      has been applied previously to thin protein films and secreted ECM proteins on 
      poly(N-isopropyl acrylamide) (polyNIPAAM) surfaces. To extract trends from within 
      the complex ToF-SIMS dataset, a multivariate analysis technique, principal 
      component analysis (PCA), was employed. Using this method, a molecular 
      fingerprint of each surface was created and separation was seen in the PCA scores 
      between the decellularized esophagus and the decellularized small intestine 
      samples. The PCA scores for the decellularized bladder sample fell between the 
      previous two decellularized samples. Protein films of common extracellular matrix 
      constituents (collagen IV, collagen I, laminin, and Matrigel) were also 
      investigated. The PCA results from these protein films were used to develop 
      qualitative hypotheses for the relationship of the key fragments identified from 
      the PCA of the decellularized ECMs.
CI  - Copyright (c) 2010 Elsevier Ltd. All rights reserved.
FAU - Barnes, Christopher A
AU  - Barnes CA
AD  - University of Washington Engineered Biomaterials, Seattle, WA 98195, USA.
FAU - Brison, Jeremy
AU  - Brison J
FAU - Michel, Roger
AU  - Michel R
FAU - Brown, Bryan N
AU  - Brown BN
FAU - Castner, David G
AU  - Castner DG
FAU - Badylak, Stephen F
AU  - Badylak SF
FAU - Ratner, Buddy D
AU  - Ratner BD
LA  - eng
GR  - P41 EB002027/EB/NIBIB NIH HHS/United States
GR  - P41 EB002027-24/EB/NIBIB NIH HHS/United States
GR  - EB-002027/EB/NIBIB NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20101104
PL  - Netherlands
TA  - Biomaterials
JT  - Biomaterials
JID - 8100316
RN  - 0 (Membrane Proteins)
SB  - IM
MH  - Animals
MH  - Basement Membrane/cytology/metabolism
MH  - Esophagus/cytology
MH  - Extracellular Matrix/*chemistry/ultrastructure
MH  - Immunohistochemistry
MH  - Intestine, Small/cytology
MH  - Male
MH  - *Materials Testing
MH  - Membrane Proteins/metabolism
MH  - Principal Component Analysis
MH  - Rats
MH  - Rats, Inbred F344
MH  - Spectrometry, Mass, Secondary Ion
MH  - Surface Properties
MH  - Urinary Bladder/cytology
PMC - PMC2997685
MID - NIHMS251633
EDAT- 2010/11/09 06:00
MHDA- 2011/02/25 06:00
PMCR- 2012/01/01
CRDT- 2010/11/09 06:00
PHST- 2010/08/17 00:00 [received]
PHST- 2010/09/02 00:00 [accepted]
PHST- 2010/11/09 06:00 [entrez]
PHST- 2010/11/09 06:00 [pubmed]
PHST- 2011/02/25 06:00 [medline]
PHST- 2012/01/01 00:00 [pmc-release]
AID - S0142-9612(10)01159-2 [pii]
AID - 10.1016/j.biomaterials.2010.09.007 [doi]
PST - ppublish
SO  - Biomaterials. 2011 Jan;32(1):137-43. doi: 10.1016/j.biomaterials.2010.09.007. 
      Epub 2010 Nov 4.