PMID- 21055951
OWN - NLM
STAT- MEDLINE
DCOM- 20110308
LR  - 20101129
IS  - 1464-3391 (Electronic)
IS  - 0968-0896 (Linking)
VI  - 18
IP  - 24
DP  - 2010 Dec 15
TI  - Effect of Reishi polysaccharides on human stem/progenitor cells.
PG  - 8583-91
LID - 10.1016/j.bmc.2010.10.016 [doi]
AB  - The polysaccharide fraction of Ganoderma lucidum (F3) was found to benefit our 
      health in many ways by influencing the activity of tissue stem/progenitor cells. 
      In this study, F3 was found to promote the adipose tissue MSCs' aggregation and 
      chondrosphere formation, with the increase of CAM (N-CAM, I-CAM) expressions and 
      autokine (BMP-2, IL-11, and aggrecan) secretions, in an in vitro chondrogenesis 
      assay. In a stem cell expansion culture, it possesses the thrombopoietin (TPO) 
      and GM-CSF like functions to enhance the survival/renewal abilities of primitive 
      hematopoietic stem/progenitor cells (HSCs). F3 was found to promote the dendrite 
      growth of blood mononuclear cells (MNCs) and the expression of cell adhesion 
      molecules in the formation of immature dendritic cells (DC). On the other hand, 
      F3 exhibited inhibitory effects on blood endothelial progenitor (EPC) colony 
      formation, with concomitant reduction of cell surface endoglin (CD105) and 
      vascular endothelial growth factor receptor-3 (VEGFR-3) marker expressions, in 
      the presence of angiogenic factors. A further cytokine array analysis revealed 
      that F3 indeed inhibited the angiogenin synthesis and enhanced IL-1, MCP-1, 
      MIP-1, RANTES, and GRO productions in the blood EPC derivation culture. 
      Collectively, we have demonstrated that the polysaccharide fraction of G. lucidum 
      F3 exhibits cytokine and chemokine like functions which are beneficial to human 
      tissue stem/progenitor cells by modulating their CAM expressions and biological 
      activities. These findings provide us a better the observation that F3 
      glycopolysaccharides indeed possesses anti-angiogenic and immune-modulating 
      functions and promotes hematopoietic stem/progenitor cell homing for better human 
      tissue protection, reducing disease progression and health.
CI  - Copyright (c) 2010 Elsevier Ltd. All rights reserved.
FAU - Chen, Wan-Yu
AU  - Chen WY
AD  - Genomics Research Center, Academia Sinica, No. 128, Academia Road, Sec. 2, 
      Nankang, Taipei 115, Taiwan.
FAU - Yang, Wen-Bin
AU  - Yang WB
FAU - Wong, Chi-Huey
AU  - Wong CH
FAU - Shih, Daniel Tzu-Bi
AU  - Shih DT
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20101104
PL  - England
TA  - Bioorg Med Chem
JT  - Bioorganic & medicinal chemistry
JID - 9413298
RN  - 0 (Cell Adhesion Molecules)
RN  - 0 (Cytokines)
RN  - 0 (Polysaccharides)
SB  - IM
MH  - Adipose Tissue/cytology
MH  - Cell Adhesion/drug effects
MH  - Cell Adhesion Molecules/genetics
MH  - Cytokines/genetics
MH  - Gene Expression Regulation/drug effects
MH  - Hematopoietic Stem Cells/*drug effects
MH  - Humans
MH  - Polysaccharides/*pharmacology
MH  - Reishi/*chemistry
EDAT- 2010/11/09 06:00
MHDA- 2011/03/09 06:00
CRDT- 2010/11/09 06:00
PHST- 2010/07/29 00:00 [received]
PHST- 2010/10/01 00:00 [revised]
PHST- 2010/10/06 00:00 [accepted]
PHST- 2010/11/09 06:00 [entrez]
PHST- 2010/11/09 06:00 [pubmed]
PHST- 2011/03/09 06:00 [medline]
AID - S0968-0896(10)00938-7 [pii]
AID - 10.1016/j.bmc.2010.10.016 [doi]
PST - ppublish
SO  - Bioorg Med Chem. 2010 Dec 15;18(24):8583-91. doi: 10.1016/j.bmc.2010.10.016. Epub 
      2010 Nov 4.