PMID- 21056723
OWN - NLM
STAT- MEDLINE
DCOM- 20110314
LR  - 20191210
IS  - 1873-4324 (Electronic)
IS  - 0003-2670 (Linking)
VI  - 682
IP  - 1-2
DP  - 2010 Dec 3
TI  - Efficient hydrophilic interaction liquid chromatography-tandem mass spectrometry 
      for the multiclass analysis of veterinary drugs in chicken muscle.
PG  - 117-29
LID - 10.1016/j.aca.2010.09.048 [doi]
AB  - A simple and sensitive method has been developed for multiresidue analysis of 24 
      important veterinary drugs (including 3 aminoglycosides, 3 beta-lactams, 2 
      lincosamides, 4 macrolides, 4 quinolones, 4 sulfonamides, 3 tetracyclines, and 
      amprolium) in chicken muscle. The method involved a simple extraction using (1:1, 
      v/v) of 2% trichloroacetic acid in water-acetonitrile, followed by removing fat 
      with hexane, dilution of sample extract, and filtration prior to liquid 
      chromatography-tandem mass spectrometric (LC-MS/MS) analysis. Hydrophilic 
      interaction liquid chromatography (HILIC) proved to be very effective for 
      separation of a wide range of polar and hydrophilic compounds (providing high 
      sensitivity and good peak shape) compared to reversed phase and ion-pair 
      separation. The method was successfully validated according to the European 
      Decision 2002/657/EC. Average recoveries were 53-99% at 0.5-MRL, MRL, and 1.5-MRL 
      spiking levels, with satisfactory precision </=15% RSD. The limit of detection of 
      the method was 0.1-10 mugkg(-1) for 22 analytes and 20 mugkg(-1) for 
      aminoglycosides. These values were lower than the maximum residue limits (MRLs) 
      established by the European Union. The evaluated method provides reliable 
      screening, quantification, and identification of 24 veterinary drug residues in 
      foods of animal origin. It has been successfully tested in real samples (such as 
      chicken muscle, shrimp, and egg).
CI  - Copyright (c) 2010 Elsevier B.V. All rights reserved.
FAU - Chiaochan, Chayada
AU  - Chiaochan C
AD  - Chromatography and Separation Research Unit, Department of Chemistry, Faculty of 
      Science, Chulalongkorn University, Phayathai, Bangkok 10330, Thailand.
FAU - Koesukwiwat, Urairat
AU  - Koesukwiwat U
FAU - Yudthavorasit, Soparat
AU  - Yudthavorasit S
FAU - Leepipatpiboon, Natchanun
AU  - Leepipatpiboon N
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Validation Study
DEP - 20101007
PL  - Netherlands
TA  - Anal Chim Acta
JT  - Analytica chimica acta
JID - 0370534
RN  - 0 (Veterinary Drugs)
SB  - IM
MH  - Animals
MH  - Chickens
MH  - Chromatography, Liquid/economics/*methods
MH  - Meat/*analysis
MH  - Muscles/chemistry
MH  - Sensitivity and Specificity
MH  - Tandem Mass Spectrometry/economics/*methods
MH  - Veterinary Drugs/*analysis
EDAT- 2010/11/09 06:00
MHDA- 2011/03/15 06:00
CRDT- 2010/11/09 06:00
PHST- 2010/08/21 00:00 [received]
PHST- 2010/09/29 00:00 [accepted]
PHST- 2010/11/09 06:00 [entrez]
PHST- 2010/11/09 06:00 [pubmed]
PHST- 2011/03/15 06:00 [medline]
AID - S0003-2670(10)01230-4 [pii]
AID - 10.1016/j.aca.2010.09.048 [doi]
PST - ppublish
SO  - Anal Chim Acta. 2010 Dec 3;682(1-2):117-29. doi: 10.1016/j.aca.2010.09.048. Epub 
      2010 Oct 7.