PMID- 21059878
OWN - NLM
STAT- MEDLINE
DCOM- 20110125
LR  - 20230526
IS  - 1098-5522 (Electronic)
IS  - 0019-9567 (Print)
IS  - 0019-9567 (Linking)
VI  - 79
IP  - 1
DP  - 2011 Jan
TI  - Differential regulation of kidney and spleen cytokine responses in mice 
      challenged with pathology-standardized doses of Candida albicans mannosylation 
      mutants.
PG  - 146-52
LID - 10.1128/IAI.01004-10 [doi]
AB  - Cell surface polysaccharides are key determinants of host responses to fungal 
      infection. We determined the effects of alterations in Candida albicans cell 
      surface polysaccharide composition and gross changes in the host immune response 
      in groups of mice challenged intravenously with five C. albicans strains at doses 
      adjusted to give equal disease progression 3 days later. The five strains used 
      were the parental strain NGY152, two mutants with defective cell wall 
      mannosylation, pmr1Delta mutant and mnt1/2Delta mutant, and the same two strains with a 
      copy of PMR1 and MNT1 reintegrated, respectively. Renal and spleen levels of 
      chemokines and cytokines previously shown to be key components of early host 
      response to C. albicans were determined at intervals up to 3 days after 
      challenge. By 12 h after C. albicans challenge, the levels of granulocyte 
      colony-stimulating factor (G-CSF), keratinocyte-derived chemokine (KC), 
      interleukin 6 (IL-6), monocyte chemotactic peptide 1 (MCP-1), macrophage 
      inflammatory protein 1alpha (MIP-1alpha), MIP-1beta, and MIP-2 were higher in the kidneys of 
      mice challenged with the pmr1Delta mutant than in animals challenged with the other 
      strains and were lower by day 3, suggesting an earlier host response to the pmr1Delta 
      mutant. The production of these chemokines also diminished earlier than controls 
      in mice infected with the mnt1/2Delta strain. Although these differences were 
      statistically significant, their magnitude was seldom great, and no unambiguous 
      evidence was obtained for individual responses specific to any cell surface 
      glycosylation change. We conclude that complex, multifactorial local responses 
      offset and obscure any differences resulting from differences in surface 
      mannosylation of C. albicans strains when infection results from 
      pathology-standardized challenges.
FAU - Castillo, Luis
AU  - Castillo L
AD  - Aberdeen Fungal Group, Institute of Medical Sciences, University of Aberdeen, 
      Aberdeen AB25 2ZD, United Kingdom.
FAU - MacCallum, Donna M
AU  - MacCallum DM
FAU - Brown, Alistair J P
AU  - Brown AJ
FAU - Gow, Neil A R
AU  - Gow NA
FAU - Odds, Frank C
AU  - Odds FC
LA  - eng
GR  - WT_/Wellcome Trust/United Kingdom
GR  - 076954/Z/05/Z/WT_/Wellcome Trust/United Kingdom
GR  - 080088/Z/06/Z/WT_/Wellcome Trust/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20101108
PL  - United States
TA  - Infect Immun
JT  - Infection and immunity
JID - 0246127
RN  - 0 (Antigens, Surface)
RN  - 0 (Polysaccharides)
SB  - IM
MH  - Animals
MH  - Antigens, Surface/genetics/*metabolism
MH  - Candida albicans/classification/*genetics
MH  - Candidiasis/immunology/microbiology
MH  - Cell Wall/chemistry/*metabolism
MH  - Female
MH  - Gene Expression Regulation, Fungal/physiology
MH  - Kidney/immunology/metabolism/pathology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mutation
MH  - Polysaccharides/chemistry/*metabolism
MH  - Spleen/immunology/metabolism
PMC - PMC3019922
EDAT- 2010/11/10 06:00
MHDA- 2011/01/28 06:00
PMCR- 2011/07/01
CRDT- 2010/11/10 06:00
PHST- 2010/11/10 06:00 [entrez]
PHST- 2010/11/10 06:00 [pubmed]
PHST- 2011/01/28 06:00 [medline]
PHST- 2011/07/01 00:00 [pmc-release]
AID - IAI.01004-10 [pii]
AID - 1004-10 [pii]
AID - 10.1128/IAI.01004-10 [doi]
PST - ppublish
SO  - Infect Immun. 2011 Jan;79(1):146-52. doi: 10.1128/IAI.01004-10. Epub 2010 Nov 8.