PMID- 21062634
OWN - NLM
STAT- MEDLINE
DCOM- 20110404
LR  - 20191210
IS  - 1872-8359 (Electronic)
IS  - 0167-7012 (Linking)
VI  - 84
IP  - 1
DP  - 2011 Jan
TI  - Comparison of four methods, including semi-automated rep-PCR, for the typing of 
      vancomycin-resistant Enterococcus faecium.
PG  - 74-80
LID - 10.1016/j.mimet.2010.10.014 [doi]
AB  - We have assessed the performance of semi-automated rep-PCR (Diversilab(R)) and 
      multilocus sequence typing (MLST) in comparison to pulsed-field gel 
      electrophoresis (PFGE) for typing a collection of 29 epidemiologically 
      characterized vancomycin-resistant Enterococcus faecium (VRE). Sixteen strains 
      that harbored the Tn1546 element were typed by PCR mapping. The discriminative 
      power of the typing methods was calculated by the Simpson's index of diversity, 
      and the concordance between methods was evaluated by the Kendall's coefficient of 
      concordance. Semi-automated rep-PCR appeared as discriminative as PFGE and was 
      further compared with PFGE for typing 67 VRE isolated during a hospital outbreak. 
      Rep-PCR appeared to be more discriminative than PFGE for this second set of 
      strains. Reproducibility of DiversiLab(R) was also tested against 35 selected 
      isolates. Only three showed less than 97% similarity, indicating high 
      reproducibility at this level of discrimination. In conclusion, semi-automated 
      rep-PCR is a useful tool for rapid screening of VRE isolates during an outbreak, 
      although cost of the system may be limiting for routine implementation. PFGE, 
      which remains the reference method, should be used for confirmation and 
      evaluation of the genetic relatedness of epidemic isolates.
CI  - Copyright (c) 2010 Elsevier B.V. All rights reserved.
FAU - Bourdon, Nancy
AU  - Bourdon N
AD  - Centre National de Reference de la Resistance aux Antibiotiques, Service de 
      Microbiologie, CHU Cote de Nacre, Universite de Caen, avenue Cote de Nacre, 14033 
      Caen Cedex, France.
FAU - Lemire, Astrid
AU  - Lemire A
FAU - Fines-Guyon, Marguerite
AU  - Fines-Guyon M
FAU - Auzou, Michel
AU  - Auzou M
FAU - Perichon, Bruno
AU  - Perichon B
FAU - Courvalin, Patrice
AU  - Courvalin P
FAU - Cattoir, Vincent
AU  - Cattoir V
FAU - Leclercq, Roland
AU  - Leclercq R
LA  - eng
PT  - Comparative Study
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20101106
PL  - Netherlands
TA  - J Microbiol Methods
JT  - Journal of microbiological methods
JID - 8306883
RN  - 0 (DNA Transposable Elements)
RN  - 0 (DNA, Bacterial)
SB  - IM
MH  - Automation/methods
MH  - Bacterial Typing Techniques/*methods
MH  - DNA Transposable Elements
MH  - DNA, Bacterial/genetics
MH  - Electrophoresis, Gel, Pulsed-Field/methods
MH  - Enterococcus faecium/*classification/*drug effects/genetics
MH  - Genetic Variation
MH  - Genotype
MH  - Gram-Positive Bacterial Infections/microbiology
MH  - Humans
MH  - Molecular Typing/*methods
MH  - Multilocus Sequence Typing/methods
MH  - Polymerase Chain Reaction/methods
MH  - Reproducibility of Results
MH  - *Vancomycin Resistance
EDAT- 2010/11/11 06:00
MHDA- 2011/04/05 06:00
CRDT- 2010/11/11 06:00
PHST- 2010/09/23 00:00 [received]
PHST- 2010/10/11 00:00 [revised]
PHST- 2010/10/21 00:00 [accepted]
PHST- 2010/11/11 06:00 [entrez]
PHST- 2010/11/11 06:00 [pubmed]
PHST- 2011/04/05 06:00 [medline]
AID - S0167-7012(10)00356-8 [pii]
AID - 10.1016/j.mimet.2010.10.014 [doi]
PST - ppublish
SO  - J Microbiol Methods. 2011 Jan;84(1):74-80. doi: 10.1016/j.mimet.2010.10.014. Epub 
      2010 Nov 6.