PMID- 21063062
OWN - NLM
STAT- MEDLINE
DCOM- 20110302
LR  - 20211020
IS  - 2190-3883 (Electronic)
IS  - 1234-1983 (Linking)
VI  - 51
IP  - 4
DP  - 2010
TI  - Variability of ribosomal DNA sites in Festuca pratensis, Lolium perenne, and 
      their intergeneric hybrids, revealed by FISH and GISH.
PG  - 449-60
LID - 10.1007/BF03208874 [doi]
AB  - This study focuses on the variability of chromosomal location and number of 
      ribosomal DNA (rDNA) sites in some diploid and autotetraploid Festuca pratensis 
      and Lolium perenne cultivars, as well as on identification of rDNA-bearing 
      chromosomes in their triploid and tetraploid F. pratensis × L. perenne 
      hybrids. The rDNA loci were mapped using fluorescence in situ hybridization 
      (FISH) with 5S and 25S rDNA probes, and the origin of parental genomes was 
      verified by genomic in situ hybridization (GISH) with L. perenne genomic DNA as a 
      probe, and F. pratensis genomic DNA as a block. FISH detected variation in the 
      number and chromosomal location of both 5S and 45S rDNA sites. In F. pratensis 
      mostly additional signals of 5S rDNA loci occurred, as compared with standard F. 
      pratensis karyotypes. Losses of 45S rDNA loci were more frequent in L. perenne 
      cultivars and intergeneric hybrids. Comparison of the F. pratensis and L. perenne 
      genomes approved a higher number of rDNA sites as well as variation in 
      chromosomal rDNA location in L. perenne. A greater instability of F. 
      pratensis-genome-like and L. perenne-genome-like chromosomes in tetraploid 
      hybrids was revealed, indicating gains and losses of rDNA loci, respectively. Our 
      data indicate that the rDNA loci physically mapped on chromosomes 2 and 3 in F. 
      pratensis and on chromosome 3 in L. perenne are useful markers for these 
      chromosomes in intergeneric Festuca × Lolium hybrids.
FAU - Ksiazczyk, T
AU  - Ksiazczyk T
AD  - Institute of Plant Genetics, Polish Academy of Sciences, Laboratory of 
      Cytogenetics and Molecular Biology, Strzeszynska 34, 60-479 Poznan, Poland. 
      tksi@igr.poznan.pl
FAU - Taciak, M
AU  - Taciak M
FAU - Zwierzykowski, Z
AU  - Zwierzykowski Z
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Appl Genet
JT  - Journal of applied genetics
JID - 9514582
RN  - 0 (DNA, Ribosomal)
SB  - IM
MH  - Chromosomes, Plant/genetics
MH  - Crosses, Genetic
MH  - DNA, Ribosomal/*genetics
MH  - Diploidy
MH  - Festuca/*genetics
MH  - *Genetic Variation
MH  - Genome, Plant/*genetics
MH  - *Hybridization, Genetic
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Lolium/*genetics
MH  - Metaphase/genetics
MH  - Tetraploidy
EDAT- 2010/11/11 06:00
MHDA- 2011/03/03 06:00
CRDT- 2010/11/11 06:00
PHST- 2010/11/11 06:00 [entrez]
PHST- 2010/11/11 06:00 [pubmed]
PHST- 2011/03/03 06:00 [medline]
AID - 594 [pii]
AID - 10.1007/BF03208874 [doi]
PST - ppublish
SO  - J Appl Genet. 2010;51(4):449-60. doi: 10.1007/BF03208874.