PMID- 21063108
OWN - NLM
STAT- MEDLINE
DCOM- 20110302
LR  - 20220318
IS  - 1421-9778 (Electronic)
IS  - 1015-8987 (Print)
IS  - 1015-8987 (Linking)
VI  - 26
IP  - 4-5
DP  - 2010
TI  - Modulation of ghrelin O-acyltransferase expression in pancreatic islets.
PG  - 707-16
LID - 10.1159/000322338 [doi]
AB  - BACKGROUND: Ghrelin, the only identified circulating orexigenic signal, is unique 
      in structure in which a specific acyl-modification of its third serine occurs. 
      This acylation is necessary for ghrelin to bind to its receptor and to exert its 
      biologic activity, which is catalyzed by ghrelin O-acyltransferase (GOAT). 
      Although ghrelin is mainly secreted from gastric X/A like endocrine cells, it is 
      also expressed in pancreatic islet cells and regulates insulin secretion. In this 
      study, we examined the expression and regulation of GOAT in pancreas. METHODS: 
      GOAT mRNA and immunoreactivity were examined in pancreatic islets and INS-1 cells 
      by RT-PCR and immunofluorescent staining or Western blotting. RESULTS: Insulin 
      inhibits the expression of GOAT mRNA and GOAT promoter activity in a dose and 
      time-dependent manner. The mammalian target of rapamycin (mTOR) is activated by 
      insulin. Blocking mTOR signaling by either rapamycin or overexpression of its 
      negative regulator tuberous sclerosis complex 1 (TSC1) or TSC2 attenuates the 
      inhibitory effect of insulin on the transcription and translation of GOAT. 
      CONCLUSION: Our study suggests that GOAT is present in pancreatic islet cells and 
      that insulin inhibits the expression of GOAT via the mediation of mTOR signaling.
CI  - Copyright (c) 2010 S. Karger AG, Basel.
FAU - An, Wenjiao
AU  - An W
AD  - Department of Physiology and Pathophysiology, Peking University Health Science 
      Center, Beijing, China.
FAU - Li, Yin
AU  - Li Y
FAU - Xu, Geyang
AU  - Xu G
FAU - Zhao, Jing
AU  - Zhao J
FAU - Xiang, Xinxin
AU  - Xiang X
FAU - Ding, Li
AU  - Ding L
FAU - Li, Jing
AU  - Li J
FAU - Guan, Youfei
AU  - Guan Y
FAU - Wang, Xian
AU  - Wang X
FAU - Tang, Chaosu
AU  - Tang C
FAU - Li, Xiaoying
AU  - Li X
FAU - Mulholland, Michael
AU  - Mulholland M
FAU - Zhang, Weizhen
AU  - Zhang W
LA  - eng
GR  - R01 DK043225/DK/NIDDK NIH HHS/United States
GR  - R01 DK054032/DK/NIDDK NIH HHS/United States
GR  - R37 DK043225/DK/NIDDK NIH HHS/United States
GR  - R01DK043225/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20101029
PL  - Germany
TA  - Cell Physiol Biochem
JT  - Cellular physiology and biochemistry : international journal of experimental 
      cellular physiology, biochemistry, and pharmacology
JID - 9113221
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (Insulin)
RN  - 0 (Tsc1 protein, mouse)
RN  - 0 (Tsc1 protein, rat)
RN  - 0 (Tuberous Sclerosis Complex 1 Protein)
RN  - 0 (Tumor Suppressor Proteins)
RN  - EC 2.3.- (Acyltransferases)
RN  - EC 2.7.1.1 (mTOR protein, rat)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
RN  - EC 3.1.- (ghrelin O-acyltransferase, rat)
RN  - W36ZG6FT64 (Sirolimus)
SB  - IM
MH  - Acyltransferases/genetics/*metabolism
MH  - Animals
MH  - Anti-Bacterial Agents/pharmacology
MH  - Insulin/pharmacology
MH  - Islets of Langerhans/*enzymology
MH  - Male
MH  - Mice
MH  - Promoter Regions, Genetic
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Signal Transduction
MH  - Sirolimus/pharmacology
MH  - TOR Serine-Threonine Kinases/metabolism
MH  - Tuberous Sclerosis Complex 1 Protein
MH  - Tumor Suppressor Proteins/metabolism
PMC - PMC3048940
EDAT- 2010/11/11 06:00
MHDA- 2011/03/03 06:00
PMCR- 2011/10/01
CRDT- 2010/11/11 06:00
PHST- 2010/08/25 00:00 [accepted]
PHST- 2010/11/11 06:00 [entrez]
PHST- 2010/11/11 06:00 [pubmed]
PHST- 2011/03/03 06:00 [medline]
PHST- 2011/10/01 00:00 [pmc-release]
AID - 000322338 [pii]
AID - cpb0026-0707 [pii]
AID - 10.1159/000322338 [doi]
PST - ppublish
SO  - Cell Physiol Biochem. 2010;26(4-5):707-16. doi: 10.1159/000322338. Epub 2010 Oct 
      29.