PMID- 2106316
OWN - NLM
STAT- MEDLINE
DCOM- 19900320
LR  - 20190612
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 166
IP  - 3
DP  - 1990 Feb 14
TI  - Dopachrome conversion factor functions as an isomerase.
PG  - 1328-33
AB  - Dopachrome conversion factor is an enzymatic activity associated with the 
      pigmentary system which catalyzes the conversion of dopachrome, an intermediate 
      in melanin biosynthesis, to dihydroxyindole-2-carboxylic acid (DHICA). To date, 
      the mechanism of action of DCF has been unknown because all previous assays have 
      employed a dopachrome substrate contaminated with L-dopa. It has therefore not 
      been possible to determine whether L-dopa acts as a hydrogen donor in the 
      reaction or whether the formation of DHICA occurs through an isomerization of 
      dopachrome. In this study it is shown that DCF catalyzes the conversion of 
      dopachrome to DHICA equally well in the presence or absence of L-dopa. The 
      DCF-mediated reaction thus appears to be an isomeric rearrangement of hydrogen 
      ions from one portion of the dopachrome molecule to another. The results indicate 
      that the name "dopachrome isomerase" appropriately describes the function of DCF.
FAU - Pawelek, J M
AU  - Pawelek JM
AD  - Department of Dermatology, Yale University School of Medicine, New Haven, CT 
      06510.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Indolequinones)
RN  - 0 (Indoles)
RN  - 0 (Quinones)
RN  - 3571-34-4 (dopachrome)
RN  - EC 1.10.3.1 (Catechol Oxidase)
RN  - EC 5.- (Isomerases)
RN  - EC 5.3.- (Intramolecular Oxidoreductases)
RN  - EC 5.3.3.12 (dopachrome isomerase)
SB  - IM
MH  - Animals
MH  - Catechol Oxidase/isolation & purification/*metabolism
MH  - Chromatography, DEAE-Cellulose
MH  - Chromatography, High Pressure Liquid
MH  - *Indolequinones
MH  - Indoles/chemical synthesis/isolation & purification
MH  - *Intramolecular Oxidoreductases
MH  - *Isomerases
MH  - Kinetics
MH  - Melanoma, Experimental/enzymology
MH  - Mice
MH  - Quinones/chemical synthesis/isolation & purification
EDAT- 1990/02/14 00:00
MHDA- 1990/02/14 00:01
CRDT- 1990/02/14 00:00
PHST- 1990/02/14 00:00 [pubmed]
PHST- 1990/02/14 00:01 [medline]
PHST- 1990/02/14 00:00 [entrez]
AID - 0006-291X(90)91011-G [pii]
AID - 10.1016/0006-291x(90)91011-g [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 1990 Feb 14;166(3):1328-33. doi: 
      10.1016/0006-291x(90)91011-g.