PMID- 21063763
OWN - NLM
STAT- MEDLINE
DCOM- 20110801
LR  - 20211020
IS  - 1572-994X (Electronic)
IS  - 0920-8569 (Linking)
VI  - 42
IP  - 1
DP  - 2011 Feb
TI  - Activation of NF-kappaB by nucleocapsid protein of the porcine reproductive and 
      respiratory syndrome virus.
PG  - 76-81
LID - 10.1007/s11262-010-0548-6 [doi]
AB  - Nuclear factor kappa B (NF-kappaB) is a critical transcription factor in innate and 
      adaptive immune response as well as cell proliferation and survival. Previous 
      studies have demonstrated that porcine reproductive and respiratory syndrome 
      virus (PRRSV) infection activated NF-kappaB pathways through IkappaB degradation in 
      MARC-145 cells and alveolar macrophages. To evaluate the mechanisms behind this, 
      we investigated the role of PRRSV structural proteins in the regulation of NF-kappaB. 
      In this study, we screened the structural proteins of PRRSV by NF-kappaB DNA-binding 
      assay and luciferase activity assay and demonstrated that PRRSV nucleocapsid (N) 
      protein could activate NF-kappaB in MARC-145 cells. Furthermore, we revealed that the 
      region between aa 30 and 73 of N protein was essential for its function in the 
      activation of NF-kappaB. These results presented here provide a basis for 
      understanding molecular mechanism of PRRSV infection and inflammation response.
FAU - Luo, Rui
AU  - Luo R
AD  - State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural 
      University, Wuhan, China.
FAU - Fang, Liurong
AU  - Fang L
FAU - Jiang, Yunbo
AU  - Jiang Y
FAU - Jin, Hui
AU  - Jin H
FAU - Wang, Yanwei
AU  - Wang Y
FAU - Wang, Dang
AU  - Wang D
FAU - Chen, Huanchun
AU  - Chen H
FAU - Xiao, Shaobo
AU  - Xiao S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20101110
PL  - United States
TA  - Virus Genes
JT  - Virus genes
JID - 8803967
RN  - 0 (NF-kappa B)
RN  - 0 (Nucleocapsid Proteins)
SB  - IM
MH  - Animals
MH  - Cell Line
MH  - Gene Expression Regulation
MH  - NF-kappa B/*metabolism
MH  - Nucleocapsid Proteins/genetics/*metabolism
MH  - Porcine respiratory and reproductive syndrome 
      virus/genetics/metabolism/*pathogenicity
MH  - Swine
EDAT- 2010/11/11 06:00
MHDA- 2011/08/02 06:00
CRDT- 2010/11/11 06:00
PHST- 2010/07/14 00:00 [received]
PHST- 2010/10/25 00:00 [accepted]
PHST- 2010/11/11 06:00 [entrez]
PHST- 2010/11/11 06:00 [pubmed]
PHST- 2011/08/02 06:00 [medline]
AID - 10.1007/s11262-010-0548-6 [doi]
PST - ppublish
SO  - Virus Genes. 2011 Feb;42(1):76-81. doi: 10.1007/s11262-010-0548-6. Epub 2010 Nov 
      10.