PMID- 21063856
OWN - NLM
STAT- MEDLINE
DCOM- 20130412
LR  - 20211020
IS  - 1672-0733 (Print)
IS  - 1672-0733 (Linking)
VI  - 30
IP  - 5
DP  - 2010 Oct
TI  - Use of dentritic cells pulsed with HLA-A2-restricted MAGE-A1 peptide to generate 
      cytotoxic T lymphocytes against malignant glioma.
PG  - 678-82
LID - 10.1007/s11596-010-0564-8 [doi]
AB  - This study developed a novel approach of targeting malignant glioma with 
      pMAGE-A1(278-286)-specific cytotoxic T lymphocytes (CTLs) induced from the 
      peripheral blood mononuclear cells of healthy donors by multiple stimulations 
      with human leukocyte antigen (HLA)-A2-restricted pMAGE-A1(278-286) peptide-pulsed 
      dentritic cells. Cytotoxic assays were performed by the colorimetric CytoTox 96 
      assay to analyze cytotoxic activity of the induced CTLs against various target 
      cells. The induced CTLs showed approximately 45% specific lysis against 
      T2pMAGE-A1(278-286) (pMAGE-A1(278-286) peptide pulsed T2 cells) and U251 
      (HLA-A2(+), MAGE-A1(+)) at an effector:target ratio of 40:1, and approximately 5% 
      cytolysis against T2pHIV, A172 (HLA-A2(-), MAGE-A1(+)), K562 and T2 cells without 
      being pulsed with peptide at any effector:target ratio. The specific killing 
      activity of the induced CTLs against T2pMAGE-A1(278-286) and U251 was much more 
      obvious than in any other control group (P<0.05). The cytotoxic activity against 
      the T2pMAGE-A1(278-286) and U251 was significantly eliminated by anti-HLA class I 
      mAb W6/32. These results suggest that pMAGE-A1(278-286) epitope may serve as a 
      surrogate tumor antigen target of specific immunotherapy for treating HLA-A2 
      patients with malignant glioma.
FAU - Shi, Haojun
AU  - Shi H
AD  - Department of Radiology, Union Hospital, Huazhong University of Science and 
      Technology, Wuhan, China. hjshi21@21cn.com
FAU - Jiang, Xiaobing
AU  - Jiang X
FAU - Fu, Peng
AU  - Fu P
FAU - Zhou, Yi
AU  - Zhou Y
FAU - Lu, Xiaoling
AU  - Lu X
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20101110
PL  - China
TA  - J Huazhong Univ Sci Technolog Med Sci
JT  - Journal of Huazhong University of Science and Technology. Medical sciences = Hua 
      zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. 
      Yixue Yingdewen ban
JID - 101169627
RN  - 0 (Antigens, Neoplasm)
RN  - 0 (Cancer Vaccines)
RN  - 0 (Epitopes, T-Lymphocyte)
RN  - 0 (HLA-A2 Antigen)
RN  - 0 (MAGEA1 protein, human)
RN  - 0 (Melanoma-Specific Antigens)
SB  - IM
MH  - Antigens, Neoplasm/immunology
MH  - Brain Neoplasms/*immunology
MH  - Cancer Vaccines/immunology
MH  - Cell Line, Tumor
MH  - Dendritic Cells/*immunology
MH  - Epitopes, T-Lymphocyte/chemistry/immunology
MH  - Glioma/*immunology
MH  - HLA-A2 Antigen/immunology
MH  - Humans
MH  - Leukocytes, Mononuclear/immunology
MH  - Melanoma-Specific Antigens/*immunology
MH  - T-Lymphocytes, Cytotoxic/*immunology
EDAT- 2010/11/11 06:00
MHDA- 2013/04/13 06:00
CRDT- 2010/11/11 06:00
PHST- 2010/08/25 00:00 [received]
PHST- 2010/11/11 06:00 [entrez]
PHST- 2010/11/11 06:00 [pubmed]
PHST- 2013/04/13 06:00 [medline]
AID - 10.1007/s11596-010-0564-8 [doi]
PST - ppublish
SO  - J Huazhong Univ Sci Technolog Med Sci. 2010 Oct;30(5):678-82. doi: 
      10.1007/s11596-010-0564-8. Epub 2010 Nov 10.