PMID- 210672
OWN - NLM
STAT- MEDLINE
DCOM- 19781018
LR  - 20181113
IS  - 0002-9440 (Print)
IS  - 1525-2191 (Electronic)
IS  - 0002-9440 (Linking)
VI  - 92
IP  - 1
DP  - 1978 Jul
TI  - Mechanisms of platelet adhesion to the basal lamina.
PG  - 99-110
AB  - The human glomerular basal lamina (HGBL) is composed of collagenous and 
      noncollagenous glycoproteins. We assessed the role played by each costituent in 
      platelet-basal-lamina interaction by selective cleavage and removal of each 
      component by clostridial collagenase or by pepsin. When noncollagenous proteins 
      are removed from HGBL, human platelets exhibit littel reactivity toward the 
      residual collagen framework of the isolated basal lamina. With the noncollagen 
      matrix of basal lamina, after removal of the bulk of the collagen, platelet 
      adhesion and spreading proceed normally in the presence of divalent cations, 
      similar to what occurs on intact basal lamina. No platelet degranulation or 
      aggregation is observed. The results indicate that the basal lamina collagen, 
      even in its native packing arrangement, lacks affinity for platelet adhesion and 
      is incapable of triggering platelet release reactions. Platelet adhesion and 
      spreading on the basal lamina appears to depend primarily on the presence of the 
      noncollagen components and to require divalent cations. The data suggest the 
      presence on platelets of receptors for basal lamina distinct from those for 
      interstitial collagens. These receptors activate a unique modulation of platelet 
      behavior, ie, adhesion and spreading without degranulation. A difference in 
      biologic function of the basal lamina and interstitial collagens is apparent in 
      these experiments.
FAU - Huang, T W
AU  - Huang TW
FAU - Benditt, E P
AU  - Benditt EP
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Am J Pathol
JT  - The American journal of pathology
JID - 0370502
RN  - 0 (Glycoproteins)
RN  - 9007-34-5 (Collagen)
RN  - 9G34HU7RV0 (Edetic Acid)
RN  - EC 3.4.23.1 (Pepsin A)
RN  - EC 3.4.24.3 (Microbial Collagenase)
SB  - IM
MH  - Collagen/*physiology
MH  - Edetic Acid/pharmacology
MH  - Endothelium/*physiology
MH  - Glycoproteins/*physiology
MH  - Humans
MH  - Microbial Collagenase/pharmacology
MH  - Pepsin A/pharmacology
MH  - *Platelet Adhesiveness/drug effects
PMC - PMC2018598
EDAT- 1978/07/01 00:00
MHDA- 1978/07/01 00:01
PMCR- 1979/01/01
CRDT- 1978/07/01 00:00
PHST- 1978/07/01 00:00 [pubmed]
PHST- 1978/07/01 00:01 [medline]
PHST- 1978/07/01 00:00 [entrez]
PHST- 1979/01/01 00:00 [pmc-release]
PST - ppublish
SO  - Am J Pathol. 1978 Jul;92(1):99-110.