PMID- 21069472
OWN - NLM
STAT- MEDLINE
DCOM- 20110628
LR  - 20220331
IS  - 1558-9307 (Electronic)
IS  - 0024-4201 (Linking)
VI  - 46
IP  - 1
DP  - 2011 Jan
TI  - Rapid quantitative analysis of lipids using a colorimetric method in a microplate 
      format.
PG  - 95-103
LID - 10.1007/s11745-010-3494-0 [doi]
AB  - A colorimetric sulfo-phospho-vanillin (SPV) method was developed for high 
      throughput analysis of total lipids. The developed method uses a reaction mixture 
      that is maintained in a 96-well microplate throughout the entire assay. The new 
      assay provides the following advantages over other methods of lipid measurement: 
      (1) background absorbance can be easily corrected for each well, (2) there is 
      less risk of handling and transferring sulfuric acid contained in reaction 
      mixtures, (3) color develops more consistently providing more accurate 
      measurement of absorbance, and (4) the assay can be used for quantitative 
      measurement of lipids extracted from a wide variety of sources. Unlike other 
      spectrophotometric approaches that use fluorescent dyes, the optimal spectra and 
      reaction conditions for the developed assay do not vary with the sample source. 
      The developed method was used to measure lipids in extracts from four strains of 
      microalgae. No significant difference was found in lipid determination when lipid 
      content was measured using the new method and compared to results obtained using 
      a macro-gravimetric method.
FAU - Cheng, Yu-Shen
AU  - Cheng YS
AD  - Department of Biological and Agricultural Engineering, University of California, 
      Davis, CA 95616, USA.
FAU - Zheng, Yi
AU  - Zheng Y
FAU - VanderGheynst, Jean S
AU  - VanderGheynst JS
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20101111
PL  - United States
TA  - Lipids
JT  - Lipids
JID - 0060450
RN  - 0 (Benzaldehydes)
RN  - 0 (Lipids)
RN  - 0 (sulfophosphovanillin)
RN  - CHI530446X (vanillin)
SB  - IM
MH  - Benzaldehydes/*chemistry
MH  - Colorimetry/*methods
MH  - Lipids/*analysis
EDAT- 2010/11/12 06:00
MHDA- 2011/06/29 06:00
CRDT- 2010/11/12 06:00
PHST- 2010/08/11 00:00 [received]
PHST- 2010/10/15 00:00 [accepted]
PHST- 2010/11/12 06:00 [entrez]
PHST- 2010/11/12 06:00 [pubmed]
PHST- 2011/06/29 06:00 [medline]
AID - 10.1007/s11745-010-3494-0 [doi]
PST - ppublish
SO  - Lipids. 2011 Jan;46(1):95-103. doi: 10.1007/s11745-010-3494-0. Epub 2010 Nov 11.