PMID- 21069560
OWN - NLM
STAT- MEDLINE
DCOM- 20110512
LR  - 20211020
IS  - 1573-4838 (Electronic)
IS  - 0957-4530 (Linking)
VI  - 22
IP  - 1
DP  - 2011 Jan
TI  - Enhanced osteogenic differentiation of cord blood-derived unrestricted somatic 
      stem cells on electrospun nanofibers.
PG  - 165-74
LID - 10.1007/s10856-010-4174-6 [doi]
AB  - A new stem cell-scaffold construct based on poly-L-lactide (PLLA) nanofibers 
      grafted with collagen (PLLA-COL) and cord blood-derived unrestricted somatic stem 
      cells (USSC) were proposed to hold promising characteristics for bone tissue 
      engineering. Fabricated nanofibers were characterized using SEM, ATR-FTIR, 
      tensile and contact angle measurements. The capacity of PLLA, plasma-treated PLLA 
      (PLLA-pl) and PLLA-COL scaffolds to support proliferation and osteogenic 
      differentiation of USSC was evaluated using MTT assay and common osteogenic 
      markers such as alkaline phosphatase (ALP) activity, calcium mineral deposition 
      and bone-related genes. All three scaffolds showed nanofibrous and porous 
      structure with suitable physical characteristics. Higher proliferation and 
      viability of USSC was observed on PLLA-COL nanofibers compared to control 
      surfaces. In osteogenic medium, ALP activity and calcium deposition exhibited the 
      highest values on PLLA-COL scaffolds on days 7 and 14. These markers were also 
      greater on PLLA and PLLA-pl compared to TCPS. Higher levels of collagen I, 
      osteonectin and bone morphogenetic protein-2 were detected on PLLA-COL compared 
      to PLLA and PLLA-pl. Runx2 and osteocalcin were also expressed continuously on 
      all scaffolds during induction. These observations suggested the enhanced 
      proliferation and osteogenic differentiation of USSC on PLLA-COL nanofiber 
      scaffolds and introduced a new combination of stem cell-scaffold constructs with 
      desired characteristics for application in bone tissue engineering.
FAU - Seyedjafari, Ehsan
AU  - Seyedjafari E
AD  - Department of Biotechnology, College of Science, University of Tehran, Tehran, 
      Iran.
FAU - Soleimani, Masoud
AU  - Soleimani M
FAU - Ghaemi, Nasser
AU  - Ghaemi N
FAU - Sarbolouki, Mohammad Nabi
AU  - Sarbolouki MN
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
DEP - 20101111
PL  - United States
TA  - J Mater Sci Mater Med
JT  - Journal of materials science. Materials in medicine
JID - 9013087
RN  - 0 (Biocompatible Materials)
RN  - 0 (Polyesters)
RN  - 0 (Polymers)
RN  - 459TN2L5F5 (poly(lactide))
RN  - 9007-34-5 (Collagen)
SB  - IM
MH  - Biocompatible Materials/chemistry/pharmacology
MH  - Cell Differentiation/drug effects
MH  - Cells, Cultured
MH  - Collagen/chemistry/*pharmacology
MH  - Electroplating/methods
MH  - Fetal Blood/cytology
MH  - Gene Expression Regulation/drug effects
MH  - Humans
MH  - Mesenchymal Stem Cells/cytology/*drug effects/metabolism/*physiology
MH  - *Nanofibers/chemistry
MH  - Osteogenesis/*drug effects/genetics
MH  - Polyesters/chemistry/*pharmacology
MH  - Polymers/chemistry/pharmacology
MH  - Tissue Engineering
MH  - Up-Regulation/drug effects
EDAT- 2010/11/12 06:00
MHDA- 2011/05/13 06:00
CRDT- 2010/11/12 06:00
PHST- 2010/06/06 00:00 [received]
PHST- 2010/10/21 00:00 [accepted]
PHST- 2010/11/12 06:00 [entrez]
PHST- 2010/11/12 06:00 [pubmed]
PHST- 2011/05/13 06:00 [medline]
AID - 10.1007/s10856-010-4174-6 [doi]
PST - ppublish
SO  - J Mater Sci Mater Med. 2011 Jan;22(1):165-74. doi: 10.1007/s10856-010-4174-6. 
      Epub 2010 Nov 11.