PMID- 21074396
OWN - NLM
STAT- MEDLINE
DCOM- 20110414
LR  - 20151119
IS  - 1873-4235 (Electronic)
IS  - 0956-5663 (Linking)
VI  - 26
IP  - 5
DP  - 2011 Jan 15
TI  - Fabrication of stable antibody-modified field effect transistors using electrical 
      activation of Schiff base cross-linkages for tumor marker detection.
PG  - 2419-25
LID - 10.1016/j.bios.2010.10.023 [doi]
AB  - In this paper, we present a method of fabricating a rigid antibody-immobilized 
      surface using electric activation of a glutaraldehyde (GA)-modified 
      aminopropylsilyl surface for stable antibody-modified field effect transistors 
      (FETs). Electric activation of the GA-modified gate surface of the FET reduces 
      Schiff bases, which are easily hydrolyzed and collapsed, formed between GA and 
      3-aminopropyltriethoxysilane, resulting in preventing the immobilized antibodies 
      from desorbing from the surface. The lack of Raman peaks that could be assigned 
      to a Schiff base after the electrical activation of the GA-modified surface 
      indicated that the electric activation had reduced the Schiff base. The use of 
      the antibody-modified FETs has three advantages for the detection of antigens: 
      increased sensitivity, distinct recognition ability, and improved 
      reproducibility. A tumor marker, alpha-fetoprotein (AFP), was quantitatively 
      detected up to a concentration of 10 ng/mL using the antibody-modified FET. The 
      detection ability of the FET accomplished a cutoff value of hepatic cancer. The 
      quantitative detection of AFP in a solution with contaminating proteins was also 
      demonstrated. This electric activation method is applicable to other 
      antibody-modified FETs.
CI  - Copyright A(c) 2010 Elsevier B.V. All rights reserved.
FAU - Hideshima, Sho
AU  - Hideshima S
AD  - Department of Applied Chemistry, Faculty of Science and Engineering, Waseda 
      University, Okubo 3-4-1, Shinjuku-ku, Tokyo 169-8555, Japan.
FAU - Sato, Ryosuke
AU  - Sato R
FAU - Kuroiwa, Shigeki
AU  - Kuroiwa S
FAU - Osaka, Tetsuya
AU  - Osaka T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20101020
PL  - England
TA  - Biosens Bioelectron
JT  - Biosensors & bioelectronics
JID - 9001289
RN  - 0 (Antibodies, Neoplasm)
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (Cross-Linking Reagents)
RN  - 0 (Gonadotropins)
RN  - 0 (Schiff Bases)
RN  - 0 (alpha-Fetoproteins)
SB  - IM
MH  - Antibodies, Neoplasm/chemistry/immunology
MH  - Biomarkers, Tumor/*analysis
MH  - Biosensing Techniques/*instrumentation
MH  - Conductometry/*instrumentation
MH  - Cross-Linking Reagents/chemistry
MH  - Equipment Design
MH  - Equipment Failure Analysis
MH  - Gonadotropins/*analysis/chemistry/immunology
MH  - Humans
MH  - Liver Neoplasms/diagnosis/*metabolism
MH  - Schiff Bases/chemistry
MH  - *Transistors, Electronic
MH  - Tumor Cells, Cultured
MH  - alpha-Fetoproteins/*analysis/chemistry/immunology
EDAT- 2010/11/16 06:00
MHDA- 2011/04/16 06:00
CRDT- 2010/11/16 06:00
PHST- 2010/07/07 00:00 [received]
PHST- 2010/10/12 00:00 [revised]
PHST- 2010/10/13 00:00 [accepted]
PHST- 2010/11/16 06:00 [entrez]
PHST- 2010/11/16 06:00 [pubmed]
PHST- 2011/04/16 06:00 [medline]
AID - S0956-5663(10)00708-6 [pii]
AID - 10.1016/j.bios.2010.10.023 [doi]
PST - ppublish
SO  - Biosens Bioelectron. 2011 Jan 15;26(5):2419-25. doi: 10.1016/j.bios.2010.10.023. 
      Epub 2010 Oct 20.