PMID- 21074528
OWN - NLM
STAT- MEDLINE
DCOM- 20110315
LR  - 20131121
IS  - 1873-3468 (Electronic)
IS  - 0014-5793 (Linking)
VI  - 585
IP  - 1
DP  - 2011 Jan 3
TI  - Lactate downregulates the glycolytic enzymes hexokinase and phosphofructokinase 
      in diverse tissues from mice.
PG  - 92-8
LID - 10.1016/j.febslet.2010.11.009 [doi]
AB  - We examined the effects of lactate on the enzymatic activity of hexokinase (HK), 
      phosphofructokinase (PFK) and pyruvate kinase (PK) in various mouse tissues. Our 
      results showed that lactate inhibited PFK activity in all the analyzed tissues. 
      This inhibitory effect was observed in skeletal muscle even in the presence of 
      insulin. Lactate directly inhibited the phosphorylation of PFK tyrosine residues 
      in skeletal muscle, an important mechanism of the enzyme activation. Moreover, 
      lactate indirectly inhibited HK activity, which resulted from its cellular 
      redistribution, here attributed to alterations of HK structure. PK activity was 
      not affected by lactate. The activity of HK and PFK is directly related to 
      glucose metabolism. Thus, it is conceivable that lactate exposure can induce 
      inhibition of glucose consumption in tissues.
CI  - Copyright (c) 2010 Federation of European Biochemical Societies. Published by 
      Elsevier B.V. All rights reserved.
FAU - Leite, Tiago C
AU  - Leite TC
AD  - Laboratorio de Enzimologia e Controle do Metabolismo (LabECoM), Departamento de 
      Farmacos, Faculdade de Farmacia, Universidade Federal do Rio de Janeiro, Rio de 
      Janeiro, Brazil.
FAU - Coelho, Raquel G
AU  - Coelho RG
FAU - Da Silva, Daniel
AU  - Da Silva D
FAU - Coelho, Wagner S
AU  - Coelho WS
FAU - Marinho-Carvalho, Monica M
AU  - Marinho-Carvalho MM
FAU - Sola-Penna, Mauro
AU  - Sola-Penna M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20101111
PL  - England
TA  - FEBS Lett
JT  - FEBS letters
JID - 0155157
RN  - 0 (Fructosediphosphates)
RN  - 0 (Hypoglycemic Agents)
RN  - 0 (Insulin)
RN  - 33X04XA5AT (Lactic Acid)
RN  - 42HK56048U (Tyrosine)
RN  - 79082-92-1 (fructose 2,6-diphosphate)
RN  - EC 2.7.1 - (Phosphofructokinases)
RN  - EC 2.7.1.1 (Hexokinase)
SB  - IM
MH  - Animals
MH  - Blotting, Western
MH  - Down-Regulation/*drug effects
MH  - Fructosediphosphates/pharmacology
MH  - Heart/drug effects
MH  - Hexokinase/*metabolism
MH  - Hypoglycemic Agents/pharmacology
MH  - Insulin/pharmacology
MH  - Kidney/drug effects/enzymology
MH  - Lactic Acid/*pharmacology
MH  - Liver/drug effects/enzymology
MH  - Male
MH  - Mice
MH  - Muscle, Skeletal/drug effects/enzymology
MH  - Myocardium/enzymology
MH  - Phosphofructokinases/*metabolism
MH  - Phosphorylation/drug effects
MH  - Tyrosine/metabolism
EDAT- 2010/11/16 06:00
MHDA- 2011/03/16 06:00
CRDT- 2010/11/16 06:00
PHST- 2010/08/11 00:00 [received]
PHST- 2010/11/04 00:00 [revised]
PHST- 2010/11/06 00:00 [accepted]
PHST- 2010/11/16 06:00 [entrez]
PHST- 2010/11/16 06:00 [pubmed]
PHST- 2011/03/16 06:00 [medline]
AID - S0014-5793(10)00905-1 [pii]
AID - 10.1016/j.febslet.2010.11.009 [doi]
PST - ppublish
SO  - FEBS Lett. 2011 Jan 3;585(1):92-8. doi: 10.1016/j.febslet.2010.11.009. Epub 2010 
      Nov 11.