PMID- 21074610
OWN - NLM
STAT- MEDLINE
DCOM- 20110722
LR  - 20211020
IS  - 1872-9738 (Electronic)
IS  - 0892-0362 (Print)
IS  - 0892-0362 (Linking)
VI  - 33
IP  - 2
DP  - 2011 Mar-Apr
TI  - Ventromedian forebrain dysgenesis follows early prenatal ethanol exposure in 
      mice.
PG  - 231-9
LID - 10.1016/j.ntt.2010.11.001 [doi]
AB  - Ethanol exposure on gestational day (GD) 7 in the mouse has previously been shown 
      to result in ventromedian forebrain deficits along with facial anomalies 
      characteristic of fetal alcohol syndrome (FAS). To further explore ethanol's 
      teratogenic effect on the ventromedian forebrain in this mouse model, scanning 
      electron microscopic and histological analyses were conducted. For this, time 
      mated C57Bl/6J mice were injected with 2.9g/kg ethanol or saline twice, at a 4h 
      interval, on their 7th day of pregnancy. On GD 12.5, 13 and 17, control and 
      ethanol-exposed specimens were collected and processed for light and scanning 
      electron microscopic analyses. Gross morphological changes present in the 
      forebrains of ethanol-exposed embryos included cerebral hemispheres that were too 
      close in proximity or rostrally united, enlarged foramina of Monro, enlarged or 
      united lateral ventricles, and varying degrees of hippocampal and ventromedian 
      forebrain deficiency. In GD 12.5 control and ethanol-exposed embryos, in situ 
      hybridization employing probes for Nkx2.1 or Fzd8 to distinguish the preoptic 
      area and medial ganglionic eminences (MGEs) from the lateral ganglionic 
      eminences, respectively, confirmed the selective loss of ventromedian tissues. 
      Immunohistochemical labeling of oligodendrocyte progenitors with Olig2, a 
      transcription factor necessary for their specification, and of GABA, an 
      inhibitory neurotransmitter, showed ethanol-induced reductions in both. To 
      investigate later consequences of ventromedian forebrain loss, MGE-derived 
      somatostatin-expressing interneurons in the subpallial region of GD 17 fetal mice 
      were examined, with results showing that the somatostatin-expressing interneurons 
      that were present were dysmorphic in the ethanol-exposed fetuses. The potential 
      functional consequences of this insult are discussed.
CI  - Copyright (c) 2010 Elsevier Inc. All rights reserved.
FAU - Godin, Elizabeth A
AU  - Godin EA
AD  - Bowles Center for Alcohol Studies, University of North Carolina, Chapel Hill, NC 
      27599-7178, USA.
FAU - Dehart, Deborah B
AU  - Dehart DB
FAU - Parnell, Scott E
AU  - Parnell SE
FAU - O'Leary-Moore, Shonagh K
AU  - O'Leary-Moore SK
FAU - Sulik, Kathleen K
AU  - Sulik KK
LA  - eng
GR  - U01 AA017124-05/AA/NIAAA NIH HHS/United States
GR  - AA007573/AA/NIAAA NIH HHS/United States
GR  - P60 AA011605-14/AA/NIAAA NIH HHS/United States
GR  - P60 AA011605/AA/NIAAA NIH HHS/United States
GR  - AA011605/AA/NIAAA NIH HHS/United States
GR  - P50 AA011605/AA/NIAAA NIH HHS/United States
GR  - AA017124/AA/NIAAA NIH HHS/United States
GR  - T32 AA007573-14/AA/NIAAA NIH HHS/United States
GR  - U01 AA017124/AA/NIAAA NIH HHS/United States
GR  - T32 AA007573/AA/NIAAA NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20101111
PL  - United States
TA  - Neurotoxicol Teratol
JT  - Neurotoxicology and teratology
JID - 8709538
RN  - 0 (Nkx2-1 protein, mouse)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Receptors, G-Protein-Coupled)
RN  - 0 (Teratogens)
RN  - 0 (Thyroid Nuclear Factor 1)
RN  - 0 (Transcription Factors)
RN  - 0 (frizzled 8 protein, mouse)
RN  - 3K9958V90M (Ethanol)
SB  - IM
MH  - Abnormalities, Drug-Induced/embryology/*etiology/metabolism/pathology
MH  - Animals
MH  - Ethanol/*toxicity
MH  - Female
MH  - Gestational Age
MH  - Immunohistochemistry
MH  - In Situ Hybridization
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Microscopy, Electron, Scanning
MH  - Nuclear Proteins/metabolism
MH  - Pregnancy
MH  - Prenatal Exposure Delayed Effects/*chemically induced/metabolism/pathology
MH  - Receptors, G-Protein-Coupled/metabolism
MH  - Teratogens/*toxicity
MH  - Thyroid Nuclear Factor 1
MH  - Transcription Factors/metabolism
MH  - Ventromedial Hypothalamic 
      Nucleus/*abnormalities/embryology/metabolism/ultrastructure
PMC - PMC3070193
MID - NIHMS253400
EDAT- 2010/11/16 06:00
MHDA- 2011/07/23 06:00
PMCR- 2012/03/01
CRDT- 2010/11/16 06:00
PHST- 2010/09/08 00:00 [received]
PHST- 2010/11/07 00:00 [accepted]
PHST- 2010/11/16 06:00 [entrez]
PHST- 2010/11/16 06:00 [pubmed]
PHST- 2011/07/23 06:00 [medline]
PHST- 2012/03/01 00:00 [pmc-release]
AID - S0892-0362(10)00195-9 [pii]
AID - 10.1016/j.ntt.2010.11.001 [doi]
PST - ppublish
SO  - Neurotoxicol Teratol. 2011 Mar-Apr;33(2):231-9. doi: 10.1016/j.ntt.2010.11.001. 
      Epub 2010 Nov 11.