PMID- 21075456
OWN - NLM
STAT- MEDLINE
DCOM- 20110518
LR  - 20110110
IS  - 1873-2534 (Electronic)
IS  - 0165-2427 (Linking)
VI  - 139
IP  - 2-4
DP  - 2011 Feb 15
TI  - Porcine blood mononuclear cell cytokine responses to PAMP molecules: comparison 
      of mRNA and protein production.
PG  - 296-302
LID - 10.1016/j.vetimm.2010.10.016 [doi]
AB  - Pathogen-associated molecular patterns (PAMPs) are conserved molecules of 
      microorganisms inducing innate immune cells to secrete distinct patterns of 
      cytokines. In veterinary species, due to a lack of specific antibodies, cytokines 
      are often monitored as expressed mRNA only. This study investigated the induction 
      of IFN-alpha, IL-12 p40, IL-1beta, TNF-alpha, IL-6 and IL-10 by PAMP-molecules [CpG 
      oligonucleotide D19 (CpG), peptidoglycan (PGN), lipopolysaccharide (LPS), 
      Pam(3)Cys and poly-U] in porcine blood mononuclear cells (BMC) within a 24 h 
      period. As expected, cytokine responses were PAMP-specific, CpG inducing IFN-alpha 
      and IL-12 p40, and PGN, LPS and Pam(3)Cys inducing varying amounts of IL-12 p40, 
      IL-1beta, TNF-alpha, IL-6 and IL-10. Surprisingly, the ssRNA-mimic poly-U induced IL-6 
      and IL-1beta only. Using CpG, PGN and LPS, the kinetics of cytokine production 
      measured as mRNA (reverse transcription (RT)-qPCR) and protein (ELISA), 
      respectively, correlated well, mRNA responses preceding protein responses. With 
      the exception of IL-1beta and IL-6, mRNA-responses were transient, whereas protein 
      responses, except for TNF-alpha, followed saturation kinetics. Remarkably, 
      LPS-induced TNF-alpha mRNA was not followed by a protein response. These results 
      provide guidelines concerning the timing and use of protein and mRNA 
      determinations for the characterization of porcine cytokine responses to PAMPs, 
      although given the low number of animals used here results are preliminary and 
      need confirmation in a larger study.
CI  - Copyright A(c) 2010 Elsevier B.V. All rights reserved.
FAU - Sorensen, Nanna S
AU  - Sorensen NS
AD  - Innate Immunology Group, Division of Veterinary Diagnostics and Research, 
      National Veterinary Institute, Technical University of Denmark, Bulowsvej 27, 
      DK-1790 Copenhagen V, Denmark. nsor@vet.dtu.dk
FAU - Skovgaard, Kerstin
AU  - Skovgaard K
FAU - Heegaard, Peter M H
AU  - Heegaard PM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20101023
PL  - Netherlands
TA  - Vet Immunol Immunopathol
JT  - Veterinary immunology and immunopathology
JID - 8002006
RN  - 0 (Cytokines)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Oligonucleotides)
RN  - 0 (Peptides)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Animals
MH  - Cells, Cultured
MH  - Cytokines/genetics/*metabolism
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Leukocytes, Mononuclear/immunology/*metabolism
MH  - Lipopolysaccharides/immunology
MH  - Oligonucleotides/*immunology
MH  - Peptides/*immunology
MH  - RNA, Messenger/genetics/*metabolism
MH  - *Swine
MH  - Time Factors
EDAT- 2010/11/16 06:00
MHDA- 2011/05/19 06:00
CRDT- 2010/11/16 06:00
PHST- 2009/12/22 00:00 [received]
PHST- 2010/09/09 00:00 [revised]
PHST- 2010/10/14 00:00 [accepted]
PHST- 2010/11/16 06:00 [entrez]
PHST- 2010/11/16 06:00 [pubmed]
PHST- 2011/05/19 06:00 [medline]
AID - S0165-2427(10)00352-1 [pii]
AID - 10.1016/j.vetimm.2010.10.016 [doi]
PST - ppublish
SO  - Vet Immunol Immunopathol. 2011 Feb 15;139(2-4):296-302. doi: 
      10.1016/j.vetimm.2010.10.016. Epub 2010 Oct 23.