PMID- 21076185
OWN - NLM
STAT- MEDLINE
DCOM- 20110323
LR  - 20211020
IS  - 1758-5090 (Electronic)
IS  - 1758-5082 (Print)
IS  - 1758-5082 (Linking)
VI  - 2
IP  - 4
DP  - 2010 Dec
TI  - Benchtop fabrication of PDMS microstructures by an unconventional 
      photolithographic method.
PG  - 045001
LID - 10.1088/1758-5082/2/4/045001 [doi]
AB  - Poly(dimethylsiloxane) (PDMS) microstructures have been widely used in 
      bio-microelectromechanical systems (bio-MEMS) for various types of analytical, 
      diagnostic and therapeutic applications. However, PDMS-based soft lithographic 
      techniques still use conventional microfabrication processes to generate a master 
      mold, which requires access to clean room facilities and costly equipment. With 
      the increasing use of these systems in various fields, the development of 
      benchtop systems for fabricating microdevices is emerging as an important 
      challenge in their widespread use. Here we demonstrate a simple, low-cost and 
      rapid method to fabricate PDMS microstructures by using micropatterned 
      poly(ethylene glycol) diacrylate (PEGDA) master molds. In this method, PEGDA 
      microstructures were patterned on a glass substrate by photolithography under 
      ambient conditions and by using simple tools. The resulting PEGDA structures were 
      subsequently used to generate PDMS microstructures by standard molding in a 
      reproducible and repeatable manner. The thickness of the PEGDA microstructures 
      was controllable from 15 to 300 microm by using commonly available spacer materials. 
      We also demonstrate the use of this method to fabricate microfluidic channels 
      capable of generating concentration gradients. In addition, we fabricated PEGDA 
      microstructures by photolithography from the light generated from commonly 
      available laminar cell culture hood. These data suggest that this approach could 
      be beneficial for fabricating low-cost PDMS-based microdevices in resource 
      limited settings.
FAU - Hwang, Chang Mo
AU  - Hwang CM
AD  - Center for Biomedical Engineering, Department of Medicine, Brigham and Women's 
      Hospital, Harvard Medical School, 65 Landsdowne Street, Cambridge, MA 02139, USA.
FAU - Sim, Woo Young
AU  - Sim WY
FAU - Lee, Seung Hwan
AU  - Lee SH
FAU - Foudeh, Amir M
AU  - Foudeh AM
FAU - Bae, Hojae
AU  - Bae H
FAU - Lee, Sang-Hoon
AU  - Lee SH
FAU - Khademhosseini, Ali
AU  - Khademhosseini A
LA  - eng
GR  - RL1 DE019024-03/DE/NIDCR NIH HHS/United States
GR  - R21 EB007249-02/EB/NIBIB NIH HHS/United States
GR  - RL1 DE019024-02/DE/NIDCR NIH HHS/United States
GR  - R01 HL092836-03/HL/NHLBI NIH HHS/United States
GR  - DE019024/DE/NIDCR NIH HHS/United States
GR  - RL1 DE019024/DE/NIDCR NIH HHS/United States
GR  - R01 HL092836-02/HL/NHLBI NIH HHS/United States
GR  - EB007249/EB/NIBIB NIH HHS/United States
GR  - HL092836/HL/NHLBI NIH HHS/United States
GR  - R21 EB007249-01/EB/NIBIB NIH HHS/United States
GR  - R21 EB007249/EB/NIBIB NIH HHS/United States
GR  - R01 HL092836/HL/NHLBI NIH HHS/United States
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20100924
PL  - England
TA  - Biofabrication
JT  - Biofabrication
JID - 101521964
RN  - 0 (Dimethylpolysiloxanes)
RN  - 0 (poly(ethylene glycol)diacrylate)
RN  - 3WJQ0SDW1A (Polyethylene Glycols)
RN  - 63148-62-9 (baysilon)
SB  - IM
MH  - Chemistry/*methods
MH  - Dimethylpolysiloxanes/*chemistry
MH  - Microchemistry/instrumentation/*methods
MH  - Photochemistry/instrumentation/*methods
MH  - Polyethylene Glycols/chemistry
PMC - PMC3005377
MID - NIHMS255694
EDAT- 2010/11/16 06:00
MHDA- 2011/03/24 06:00
PMCR- 2010/12/21
CRDT- 2010/11/16 06:00
PHST- 2010/11/16 06:00 [entrez]
PHST- 2010/11/16 06:00 [pubmed]
PHST- 2011/03/24 06:00 [medline]
PHST- 2010/12/21 00:00 [pmc-release]
AID - S1758-5082(10)54775-4 [pii]
AID - 10.1088/1758-5082/2/4/045001 [doi]
PST - ppublish
SO  - Biofabrication. 2010 Dec;2(4):045001. doi: 10.1088/1758-5082/2/4/045001. Epub 
      2010 Sep 24.