PMID- 21078398
OWN - NLM
STAT- MEDLINE
DCOM- 20110420
LR  - 20110103
IS  - 1095-9947 (Electronic)
IS  - 1050-4648 (Linking)
VI  - 30
IP  - 1
DP  - 2011 Jan
TI  - Subcellular components of probiotics Kocuria SM1 and Rhodococcus SM2 induce 
      protective immunity in rainbow trout (Oncorhynchus mykiss, Walbaum) against 
      Vibrio anguillarum.
PG  - 347-53
LID - 10.1016/j.fsi.2010.11.005 [doi]
AB  - The efficacy of cellular components of probiotics Kocuria SM1 and Rhodococcus SM2 
      to protect rainbow trout (Oncorhynchus mykiss, Walbaum) against vibriosis was 
      assessed. Groups of fish (average weight = 10-15 g) were immunized 
      intraperitoneally (i.p.) with 0.1 ml of subcellular materials, i.e., 0.2 +/- 0.05 
      mg protein per fish, comprising extracellular proteins (ECPs), cell wall proteins 
      (CWPs) and whole cell proteins (WCPs) of SM1 and SM2, respectively, or with 0.1 
      ml of phosphate-buffered saline (PBS) to serve as the control. Seven days after 
      administration, fish from each group were challenged i.p. with 0.1 ml of a 
      suspension in PBS of 3 x 10(5) cells ml(-1) per fish of Vibrio anguillarum. Use 
      of CWPs and WCPs demonstrated significantly (P < 0.05) better protection against 
      V. anguillarum insofar as mortalities were reduced to 11-17% [relative percent 
      survival (RPS) = 80-87%], although ECPs fared less well (mortalities = 33-38%; 
      RPS = 56-62%; P > 0.05), compared to 86% mortalities of the controls. The mode of 
      action reflected activation of innate immune factors by CWPs and WCPs, 
      demonstrating significantly (P < 0.05) increased expression of respiratory burst 
      (optical density; OD(550 nm)) from 0.039 to 0.043-0.045, peroxidase (OD(550 nm)) 
      from 0.26 to 0.37-0.55, and bacterial killing activities (i.e., percentage of 
      surviving bacteria reduced from 79% to 56-57% for SM2). Moreover, an elevation of 
      leucocyte number (from 1.93% to 1.98-2.93%; P > 0.05) and immunoglubolin level 
      (from 27 mg ml(-1) to 28.5-33 mg ml(-1); P > 0.05) were observed with the 
      experimental groups. These results indicate that cell components of the 
      probiotics stimulate an immune response.
CI  - Copyright (c) 2010 Elsevier Ltd. All rights reserved.
FAU - Sharifuzzaman, S M
AU  - Sharifuzzaman SM
AD  - School of Life Sciences, Heriot-Watt University, Edinburgh EH14 4AS, UK, 
      Scotland, UK.
FAU - Abbass, A
AU  - Abbass A
FAU - Tinsley, J W
AU  - Tinsley JW
FAU - Austin, B
AU  - Austin B
LA  - eng
PT  - Controlled Clinical Trial
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20101113
PL  - England
TA  - Fish Shellfish Immunol
JT  - Fish & shellfish immunology
JID - 9505220
SB  - IM
MH  - Animals
MH  - Fish Diseases/microbiology/*prevention & control
MH  - *Oncorhynchus mykiss
MH  - Probiotics/*pharmacology
MH  - Respiratory Burst
MH  - Rhodococcus/*cytology
MH  - Subcellular Fractions
MH  - Vibrio/*classification
MH  - Vibrio Infections/prevention & control/*veterinary
EDAT- 2010/11/17 06:00
MHDA- 2011/04/22 06:00
CRDT- 2010/11/17 06:00
PHST- 2010/07/21 00:00 [received]
PHST- 2010/10/21 00:00 [revised]
PHST- 2010/11/04 00:00 [accepted]
PHST- 2010/11/17 06:00 [entrez]
PHST- 2010/11/17 06:00 [pubmed]
PHST- 2011/04/22 06:00 [medline]
AID - S1050-4648(10)00347-5 [pii]
AID - 10.1016/j.fsi.2010.11.005 [doi]
PST - ppublish
SO  - Fish Shellfish Immunol. 2011 Jan;30(1):347-53. doi: 10.1016/j.fsi.2010.11.005. 
      Epub 2010 Nov 13.