PMID- 21080783
OWN - NLM
STAT- MEDLINE
DCOM- 20110317
LR  - 20211020
IS  - 1538-2443 (Electronic)
IS  - 1355-0284 (Linking)
VI  - 16
IP  - 6
DP  - 2010 Nov
TI  - A bovine herpesvirus type 1 mutant virus with truncated glycoprotein E 
      cytoplasmic tail has defective anterograde neuronal transport in rabbit dorsal 
      root ganglia primary neuronal cultures in a microfluidic chamber system.
PG  - 457-65
AB  - Bovine herpesvirus type 1 (BHV-1) is an important component of the bovine 
      respiratory disease complex (BRDC) in cattle. Following primary intranasal and 
      ocular infection of cattle, BHV-1 establishes lifelong latent infection in 
      trigeminal ganglia (TG). Upon reactivation from latency, the virus is transported 
      from neuronal cell bodies in the TG to projected nerve endings in nose and cornea 
      of latently infected cattle where the virus shedding occurs. This property of 
      BHV-1 plays a significant role in the pathogenesis of BRDC and maintenance of 
      BHV-1 in the cattle population. Recently, we have reported that a glycoprotein E 
      (gE) cytoplasmic tail-truncated BHV-1 (BHV-1 gEAm453) did not reactivate from 
      latency and was not shed in the nasal and ocular secretions of calves and 
      rabbits. Here we describe the methods to establish rabbit primary dorsal root 
      ganglia (DRG) neuron cultures in a microfluidic chamber system and to 
      characterize in vitro anterograde and retrograde axonal transport properties of 
      BHV-1 gE-deleted and BHV-1 cytoplasmic tail-truncated gEAm453 mutant viruses 
      relative to BHV-1 gEAm453-rescued/wild-type viruses. The results clearly 
      demonstrated that whereas the BHV-1 gE-deleted, BHV-1 gEAm453, and BHV-1 
      gEAm453-rescued/wild-type viruses were transported equally efficiently in the 
      retrograde direction, only the BHV-1 gEAm453-rescued/wild-type virus was 
      transported anterogradely. Therefore, we have concluded that sequences within the 
      BHV-1 gE cytoplasmic tail are essential for anterograde axonal transport and that 
      primary rabbit DRG neuronal cultures in the microfluidic chambers are suitable 
      for BHV-1 neuronal transport studies.
FAU - Chowdhury, S I
AU  - Chowdhury SI
AD  - Department of Pathobiological Sciences, School of Veterinary Medicine, Louisiana 
      State University, Baton Rouge, Louisiana 70803, USA. Chowdh@lsu.edu
FAU - Coats, J
AU  - Coats J
FAU - Neis, R A
AU  - Neis RA
FAU - Navarro, S M
AU  - Navarro SM
FAU - Paulsen, D B
AU  - Paulsen DB
FAU - Feng, J-M
AU  - Feng JM
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20101116
PL  - United States
TA  - J Neurovirol
JT  - Journal of neurovirology
JID - 9508123
RN  - 0 (Glycoproteins)
RN  - 0 (Viral Envelope Proteins)
RN  - 0 (Viral Proteins)
RN  - 0 (Viral Tail Proteins)
RN  - 0 (bovine herpesvirus type-1 glycoproteins)
SB  - IM
MH  - Animals
MH  - Bovine Respiratory Disease Complex/virology
MH  - Cattle
MH  - Cattle Diseases/virology
MH  - Cells, Cultured
MH  - Dogs
MH  - Female
MH  - Ganglia, Spinal/metabolism/*virology
MH  - Glycoproteins/genetics/metabolism
MH  - Herpesvirus 1, Bovine/genetics/metabolism/*physiology
MH  - Neurons/cytology/*virology
MH  - Rabbits
MH  - Trigeminal Ganglion/virology
MH  - Viral Envelope Proteins/genetics/metabolism
MH  - Viral Proteins/genetics/*metabolism
MH  - Viral Tail Proteins/*metabolism
MH  - Virus Activation/genetics
MH  - Virus Latency/genetics
EDAT- 2010/11/18 06:00
MHDA- 2011/03/18 06:00
CRDT- 2010/11/18 06:00
PHST- 2010/11/18 06:00 [entrez]
PHST- 2010/11/18 06:00 [pubmed]
PHST- 2011/03/18 06:00 [medline]
AID - 10.1007/BF03210851 [doi]
PST - ppublish
SO  - J Neurovirol. 2010 Nov;16(6):457-65. doi: 10.1007/BF03210851. Epub 2010 Nov 16.