PMID- 21080873
OWN - NLM
STAT- MEDLINE
DCOM- 20110309
LR  - 20101118
IS  - 1465-3931 (Electronic)
IS  - 0031-3025 (Linking)
VI  - 42
IP  - 7
DP  - 2010 Dec
TI  - Cytogenetic findings in Wilms' tumour: a single institute study.
PG  - 643-9
LID - 10.3109/00313025.2010.522171 [doi]
AB  - AIMS: Cytogenetic abnormalities of Wilms' tumour (WT) treated in a single 
      institution in Western Australia were reviewed. Correlation with histologic 
      subtypes, stage, presence of nephrogenic rests and age of the patient at 
      diagnosis were also evaluated. METHODS: 53 WT specimens were encountered between 
      1995 and 2009. Tissue culture was obtained in 49 (92%) specimens. Reports 
      documenting histopathological features of the tumour, stage and outcome were also 
      retrieved. RESULTS: A total of 53 tumour specimens from 42 patients/cases were 
      examined and staged in accordance with the National Wilms' Tumor Study (NWTS). 
      Thirty-eight cases were unilateral (34 unifocal, 4 multifocal) and four were 
      bilateral (2 multifocal). Fifty tumours showed favourable histology WT. One 
      tumour was a cystic partially differentiated nephroblastoma (CPDN). Two tumours 
      showed diffuse anaplasia. Eighteen specimens had nephrogenic rests, seven with 
      perilobar rests, 10 with intralobar rests and one with both types of nephrogenic 
      rests. Twelve WTs were assigned as stage 1, 22 as stage 2, 16 as stage 3, and two 
      each for stages 4 and 5. For chromosomal analysis, 92% of the specimens yielded 
      results, of which 70% showed abnormal karyotype and 22% displayed normal 
      karyotypic findings. Hyperdiploidy was more common than hypodiploidy. The most 
      common chromosomal gain involved chromosome 12. Low stage tumours tended to have 
      abnormal karyotypes with hyperdiploidy and hypodiploidy being more common. There 
      was no statistical correlation between abnormal karyotype and stage or abnormal 
      karyotype and age group or abnormal karyotype and non-blastemal WT. Eleven (58%) 
      tumours harbouring nephrogenic rests displayed an abnormal karyotype. 16q loss or 
      der(16)t(1;16) were more common in younger patients but no association was made 
      between this chromosomal abnormality and stage. Monosomy 22 and gain of 1q were 
      more common in older patients. Furthermore, monosomy 22 tended to occur in 
      tumours of earlier stage. No correlation between 11p deletions and age or stage 
      was seen. CONCLUSIONS: WTs are karyotypically heterogeneous tumours. Conventional 
      cytogenetic analysis of WTs still remains a useful technique to assist in the 
      understanding of these tumours.
FAU - MdZin, Reena
AU  - MdZin R
AD  - School of Pathology and Laboratory Medicine, University of Western Australia, 
      Australia.
FAU - Murch, Ashleigh
AU  - Murch A
FAU - Charles, Adrian
AU  - Charles A
LA  - eng
PT  - Journal Article
PL  - England
TA  - Pathology
JT  - Pathology
JID - 0175411
SB  - IM
MH  - Age Factors
MH  - Child
MH  - Child, Preschool
MH  - Chromosome Aberrations
MH  - Cytogenetic Analysis
MH  - Female
MH  - *Genes, Wilms Tumor
MH  - Humans
MH  - Infant
MH  - Kidney Neoplasms/*genetics/*pathology
MH  - Male
MH  - Neoplasm Staging
MH  - Retrospective Studies
MH  - Western Australia
MH  - Wilms Tumor/*genetics/*pathology
EDAT- 2010/11/18 06:00
MHDA- 2011/03/10 06:00
CRDT- 2010/11/18 06:00
PHST- 2010/11/18 06:00 [entrez]
PHST- 2010/11/18 06:00 [pubmed]
PHST- 2011/03/10 06:00 [medline]
AID - 10.3109/00313025.2010.522171 [doi]
PST - ppublish
SO  - Pathology. 2010 Dec;42(7):643-9. doi: 10.3109/00313025.2010.522171.