PMID- 21081284
OWN - NLM
STAT- MEDLINE
DCOM- 20110414
LR  - 20110128
IS  - 1347-4421 (Electronic)
IS  - 1347-4421 (Linking)
VI  - 111
IP  - 2
DP  - 2011 Feb
TI  - Integrated lysis procedures reduce extraction biases of microbial DNA from 
      mangrove sediment.
PG  - 153-7
LID - 10.1016/j.jbiosc.2010.10.006 [doi]
AB  - Sufficient lysis of soil or sediment microbes is a critical step for analyzing 
      microbial community structures and for preparing metagenomic DNA libraries. The 
      present study compared lysis methods for recovering archaeal, bacterial, 
      actinomycete, and fungal DNAs from a mangrove sediment sample. PCR results showed 
      that individual procedures using SDS, lysozyme, sonication, freeze-thaw, 
      microwave, and vigorous shaking could extract archaeal or bacterial DNA but 
      failed for actinomycetes or fungi cells. In comparison, an integrated lysis 
      procedure using SDS, lysozyme, and vigorous shaking successfully obtained fungal 
      DNA, and a combination of SDS, lysozyme, vigorous shaking, and microwave 
      treatments recovered DNA from actinomycetes. Denaturing gradient gel 
      electrophoresis (DGGE) results showed that although single lysis procedures can 
      lyse bacterial DNA, all of them assessed the indigenous bacterial community 
      structure with significant biases. The integrated lysis protocols described in 
      the present study could be useful for extracting DNA from various types of 
      sediments.
CI  - Copyright (c) 2010 The Society for Biotechnology, Japan. Published by Elsevier B.V. 
      All rights reserved.
FAU - Jiang, Yun-Xia
AU  - Jiang YX
AD  - Department of Environmental Health, School of Public Health and Tropical 
      Medicine, Southern Medical University, Guangzhou 510515, PR China.
FAU - Wu, Ji-Guo
AU  - Wu JG
FAU - Yu, Ke-Qiang
AU  - Yu KQ
FAU - Ai, Chun-Xiang
AU  - Ai CX
FAU - Zou, Fei
AU  - Zou F
FAU - Zhou, Hong-Wei
AU  - Zhou HW
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20101116
PL  - Japan
TA  - J Biosci Bioeng
JT  - Journal of bioscience and bioengineering
JID - 100888800
RN  - 0 (DNA, Archaeal)
RN  - 0 (DNA, Bacterial)
RN  - 0 (DNA, Fungal)
SB  - IM
MH  - DNA, Archaeal/*isolation & purification
MH  - DNA, Bacterial/*isolation & purification
MH  - DNA, Fungal/*isolation & purification
MH  - Denaturing Gradient Gel Electrophoresis/methods
MH  - Geologic Sediments/*microbiology
MH  - Polymerase Chain Reaction
MH  - Water Microbiology
EDAT- 2010/11/18 06:00
MHDA- 2011/04/16 06:00
CRDT- 2010/11/18 06:00
PHST- 2010/07/26 00:00 [received]
PHST- 2010/09/21 00:00 [revised]
PHST- 2010/10/08 00:00 [accepted]
PHST- 2010/11/18 06:00 [entrez]
PHST- 2010/11/18 06:00 [pubmed]
PHST- 2011/04/16 06:00 [medline]
AID - S1389-1723(10)00334-8 [pii]
AID - 10.1016/j.jbiosc.2010.10.006 [doi]
PST - ppublish
SO  - J Biosci Bioeng. 2011 Feb;111(2):153-7. doi: 10.1016/j.jbiosc.2010.10.006. Epub 
      2010 Nov 16.