PMID- 21082320
OWN - NLM
STAT- MEDLINE
DCOM- 20111215
LR  - 20110621
IS  - 1432-069X (Electronic)
IS  - 0340-3696 (Linking)
VI  - 303
IP  - 5
DP  - 2011 Jul
TI  - Plasma cluster ions decrease the antigenicity of mite allergens and suppress 
      atopic dermatitis in NC/Nga mice.
PG  - 367-70
LID - 10.1007/s00403-010-1095-7 [doi]
AB  - Mite antigens play important roles in the pathogenesis of atopic dermatitis (AD). 
      We recently developed a novel air cleaner (KC-850U) using charged plasma cluster 
      ions to eliminate a variety of allergens from house environments. The present 
      work demonstrates the ability of KC-850U to decrease the symptoms of AD induced 
      by mite allergens. Pooled sera from the conventional NC/Nga mice, and AD model 
      animals, were incubated with varying concentrations of the control and 
      KC-850U-pretreated allergens extracted from mite. The incubated mixtures were 
      transferred to wells coated with intact allergens and subjected to ELISA to 
      measure the amounts of immunoglobulin E (IgE) bound to the wells. Kinetic 
      analysis revealed that exposure of mite extracts to plasma cluster ions 
      destructed about 95% of the epitopes of the allergens. The specific pathogen-free 
      and conventional mice were housed in rooms equipped with either KC-850U or a 
      standard air cleaner and observed their dermal symptom for 2 weeks. 
      Dermatological examination revealed the AD symptom of the conventional mice 
      housed in a room equipped with an air cleaner. In contrast, the symptoms which 
      became apparent during the experiments were suppressed remarkably exposing mice 
      to plasma cluster ions. These observations suggested that plasma cluster ions 
      generated by KC-850U destroyed the epitopes of mite allergens and suppressed the 
      symptoms of AD in the mice.
FAU - Hiramoto, Keiichi
AU  - Hiramoto K
AD  - Department of Biochemistry and Molecular Pathology, Osaka City University Medical 
      School, Asahimachi, Abeno, Japan. hiramoto@msic.med.osaka-cu.ac.jp
FAU - Orita, Kumi
AU  - Orita K
FAU - Yamate, Yurika
AU  - Yamate Y
FAU - Sato, Eisuke F
AU  - Sato EF
FAU - Okano, Hiroaki
AU  - Okano H
FAU - Nishikawa, Kazuo
AU  - Nishikawa K
FAU - Inoue, Masayasu
AU  - Inoue M
LA  - eng
PT  - Journal Article
DEP - 20101117
PL  - Germany
TA  - Arch Dermatol Res
JT  - Archives of dermatological research
JID - 8000462
RN  - 0 (Air Pollutants)
RN  - 0 (Antigens, Dermatophagoides)
RN  - 0 (Plasma Gases)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - *Air Conditioning
MH  - Air Pollutants
MH  - Animals
MH  - Antigens, Dermatophagoides/chemistry/*immunology
MH  - Dermatitis, Atopic/*immunology/*pathology
MH  - Disease Models, Animal
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Filtration
MH  - Immunoglobulin E/analysis/blood/immunology
MH  - Male
MH  - Mice
MH  - Mites/*immunology
MH  - Plasma Gases/*chemistry
EDAT- 2010/11/18 06:00
MHDA- 2011/12/16 06:00
CRDT- 2010/11/18 06:00
PHST- 2010/09/09 00:00 [received]
PHST- 2010/11/02 00:00 [accepted]
PHST- 2010/10/29 00:00 [revised]
PHST- 2010/11/18 06:00 [entrez]
PHST- 2010/11/18 06:00 [pubmed]
PHST- 2011/12/16 06:00 [medline]
AID - 10.1007/s00403-010-1095-7 [doi]
PST - ppublish
SO  - Arch Dermatol Res. 2011 Jul;303(5):367-70. doi: 10.1007/s00403-010-1095-7. Epub 
      2010 Nov 17.