PMID- 21082382
OWN - NLM
STAT- MEDLINE
DCOM- 20110601
LR  - 20211020
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Print)
IS  - 1064-3745 (Linking)
VI  - 686
DP  - 2011
TI  - Zebrafish model of the blood-brain barrier: morphological and permeability 
      studies.
PG  - 371-8
LID - 10.1007/978-1-60761-938-3_18 [doi]
AB  - The blood-brain barrier (BBB) is a monolayer of endothelial cells that is 
      regulated by the proximity of a unique basement membrane and a tightly controlled 
      molecular interaction between specialized subsets of cells including pericytes, 
      astrocytes, and neurons. Working together, these cells form a neurovascular unit 
      (NVU) that is dedicated to the local regulation of vascular function in the brain 
      and BBB integrity. Accordingly, the intrinsic complexity of the cell-matrix-cell 
      interactions of the NVU has made analyzing gene function in cell culture, tissue 
      explants, and even animal models difficult and the inability to study gene 
      function in the BBB in vivo has been a critical hurdle to advancing BBB 
      research.Zebrafish has emerged as a premier vertebrate organism to model and 
      analyze complex cellular interactions in vivo and genetic mechanisms of embryonic 
      development. To this end, we provide a technical overview of the procedures that 
      can be used in Zebrafish to analyze BBB integrity with a focus on the 
      cerebrovasculature of adult fish where the BBB is now defined. The techniques 
      that are used to measure the functional integrity, the cell biology, and the 
      ultrastructure of the BBB include permeability assays, fluorescent imaging of 
      reporter genes, and electron microscopy, respectively. Each can be applied to the 
      functional analysis of mutant fish in ways that characterize the molecular 
      sequelae to pathological insults that compromise BBB integrity. Due to the highly 
      conserved nature of both the genetics and cell biology of zebrafish when compared 
      with higher vertebrates, drug discovery techniques can be used in zebrafish 
      models to complement drug development studies in other model systems.
FAU - Eliceiri, Brian P
AU  - Eliceiri BP
AD  - Department of Surgery, University of California San Diego, San Diego, CA, USA.
FAU - Gonzalez, Ana Maria
AU  - Gonzalez AM
FAU - Baird, Andrew
AU  - Baird A
LA  - eng
GR  - R21 NS058776/NS/NINDS NIH HHS/United States
GR  - P20 GM078421-05/GM/NIGMS NIH HHS/United States
GR  - P20 GM078421/GM/NIGMS NIH HHS/United States
GR  - R01 HL073396/HL/NHLBI NIH HHS/United States
GR  - R01 CA170140/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
SB  - IM
MH  - Animals
MH  - Blood-Brain Barrier/*cytology/*physiology
MH  - Endothelial Cells/cytology/metabolism
MH  - *Models, Animal
MH  - Permeability
MH  - Zebrafish/*physiology
PMC - PMC4222041
MID - NIHMS359198
EDAT- 2010/11/18 06:00
MHDA- 2011/06/02 06:00
PMCR- 2014/11/06
CRDT- 2010/11/18 06:00
PHST- 2010/11/18 06:00 [entrez]
PHST- 2010/11/18 06:00 [pubmed]
PHST- 2011/06/02 06:00 [medline]
PHST- 2014/11/06 00:00 [pmc-release]
AID - 10.1007/978-1-60761-938-3_18 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2011;686:371-8. doi: 10.1007/978-1-60761-938-3_18.