PMID- 21083617
OWN - NLM
STAT- MEDLINE
DCOM- 20110324
LR  - 20221207
IS  - 1600-0722 (Electronic)
IS  - 0909-8836 (Print)
IS  - 0909-8836 (Linking)
VI  - 118
IP  - 6
DP  - 2010 Dec
TI  - Serotonin and fluoxetine receptors are expressed in enamel organs and LS8 cells 
      and modulate gene expression in LS8 cells.
PG  - 566-73
LID - 10.1111/j.1600-0722.2010.00778.x [doi]
AB  - The selective serotonin re-uptake inhibitor (SSRI) fluoxetine is widely used in 
      the treatment of depression in children and fertile women, but its effect on 
      developing tissues has been sparsely investigated. The aim of this study was to 
      investigate if enamel organs and ameloblast-derived cells express serotonin 
      receptors that are affected by peripherally circulating serotonin or fluoxetine. 
      Using RT-PCR and western blot analysis we found that enamel organs from 3-d-old 
      mice and ameloblast-like cells (LS8 cells) express functional serotonin 
      receptors, the rate-limiting enzyme in serotonin synthesis (Thp1), as well as the 
      serotonin transporter (5HTT), indicating that enamel organs and ameloblasts are 
      able to respond to serotonin and regulate serotonin availability. Fluoxetine and 
      serotonin enhanced the alkaline phosphatase activity in the cell culture medium 
      from cultured LS8 cells, whereas the expression of enamelin (Enam), amelogenin 
      (Amel), and matrix metalloproteinase-20 (MMP-20) were all significantly 
      down-regulated. The secretion of vascular endothelial growth factor (VEGF), 
      monocyte chemotactic protein 1 (MCP-1), and interferon-inducible protein 10 
      (IP-10) was also reduced compared with controls. In conclusion, enamel organs and 
      ameloblast-like cells express functional serotonin receptors. Reduced 
      transcription of enamel proteins and secretion of vascular factors may indicate 
      possible adverse effects of fluoxetine on amelogenesis.
CI  - (c) 2010 Eur J Oral Sci.
FAU - Riksen, Elisabeth A
AU  - Riksen EA
AD  - Department of Biomaterials, Faculty of Dentistry, University of Oslo, Oslo, 
      Norway.
FAU - Stunes, Astrid K
AU  - Stunes AK
FAU - Kalvik, Anne
AU  - Kalvik A
FAU - Gustafsson, Bjorn I
AU  - Gustafsson BI
FAU - Snead, Malcolm L
AU  - Snead ML
FAU - Syversen, Unni
AU  - Syversen U
FAU - Lyngstadaas, Stale P
AU  - Lyngstadaas SP
FAU - Reseland, Janne E
AU  - Reseland JE
LA  - eng
GR  - R01 DE006988/DE/NIDCR NIH HHS/United States
GR  - R01 DE013045/DE/NIDCR NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100930
PL  - England
TA  - Eur J Oral Sci
JT  - European journal of oral sciences
JID - 9504563
RN  - 0 (Ambn protein, mouse)
RN  - 0 (Amelogenin)
RN  - 0 (Ccl2 protein, mouse)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chemokine CXCL10)
RN  - 0 (Culture Media)
RN  - 0 (Dental Enamel Proteins)
RN  - 0 (Receptors, Serotonin)
RN  - 0 (Serotonin Plasma Membrane Transport Proteins)
RN  - 0 (Serotonin Receptor Agonists)
RN  - 0 (Serotonin Uptake Inhibitors)
RN  - 0 (Slc6a4 protein, mouse)
RN  - 0 (Vascular Endothelial Growth Factor A)
RN  - 0 (tuftelin)
RN  - 0 (vascular endothelial growth factor A, mouse)
RN  - 01K63SUP8D (Fluoxetine)
RN  - 333DO1RDJY (Serotonin)
RN  - EC 1.1.1.27 (L-Lactate Dehydrogenase)
RN  - EC 1.14.16.4 (Tph1 protein, mouse)
RN  - EC 1.14.16.4 (Tryptophan Hydroxylase)
RN  - EC 3.1.3.1 (Alkaline Phosphatase)
RN  - EC 3.4.24.- (Matrix Metalloproteinase 20)
RN  - EC 3.4.24.- (Mmp20 protein, mouse)
SB  - IM
MH  - Alkaline Phosphatase/analysis/drug effects
MH  - Ameloblasts/*drug effects
MH  - Amelogenin/analysis/drug effects
MH  - Animals
MH  - Cell Culture Techniques
MH  - Cell Line
MH  - Chemokine CCL2/analysis/drug effects
MH  - Chemokine CXCL10/analysis/drug effects
MH  - Culture Media
MH  - Dental Enamel Proteins/analysis/drug effects
MH  - Enamel Organ/*drug effects
MH  - Fluoxetine/*pharmacology
MH  - Gene Expression Regulation/drug effects
MH  - L-Lactate Dehydrogenase/analysis/drug effects
MH  - Matrix Metalloproteinase 20/analysis/drug effects
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Receptors, Serotonin/analysis/*drug effects
MH  - Serotonin/pharmacology
MH  - Serotonin Plasma Membrane Transport Proteins/analysis/drug effects
MH  - Serotonin Receptor Agonists/pharmacology
MH  - Selective Serotonin Reuptake Inhibitors/*pharmacology
MH  - Spectrophotometry, Atomic
MH  - Tryptophan Hydroxylase/analysis/drug effects
MH  - Vascular Endothelial Growth Factor A/analysis/drug effects
PMC - PMC6422030
MID - NIHMS692581
EDAT- 2010/11/19 06:00
MHDA- 2011/03/25 06:00
PMCR- 2019/03/18
CRDT- 2010/11/19 06:00
PHST- 2010/11/19 06:00 [entrez]
PHST- 2010/11/19 06:00 [pubmed]
PHST- 2011/03/25 06:00 [medline]
PHST- 2019/03/18 00:00 [pmc-release]
AID - 10.1111/j.1600-0722.2010.00778.x [doi]
PST - ppublish
SO  - Eur J Oral Sci. 2010 Dec;118(6):566-73. doi: 10.1111/j.1600-0722.2010.00778.x. 
      Epub 2010 Sep 30.