PMID- 21083857
OWN - NLM
STAT- MEDLINE
DCOM- 20110330
LR  - 20101216
IS  - 1365-294X (Electronic)
IS  - 0962-1083 (Linking)
VI  - 20
IP  - 1
DP  - 2011 Jan
TI  - Molecular analysis of parasitoid linkages (MAPL): gut contents of adult 
      parasitoid wasps reveal larval host.
PG  - 179-86
LID - 10.1111/j.1365-294X.2010.04918.x [doi]
AB  - Metamorphosing insects often have complex and poorly known life histories. In 
      particular, what they feed on during their larval stages remains unknown for the 
      vast majority of species, and its documentation only results from difficult and 
      time-intensive field observations, rearing or dissections. Through the 
      application of a DNA analysis of gut contents in adult parasitoid wasps, we were 
      able to selectively sequence a diagnostic DNA marker that permitted the 
      identification of the host used by these wasps during their larval stages. By 
      reproducing these results in species with different life histories, we excluded 
      other potential sources of host DNA, confirming that after ingestion by the 
      parasitoid larva the host DNA can persist through metamorphosis in the abdominal 
      contents of the adult wasp. Our discovery considerably extends the applicability 
      of molecular analysis of gut contents by enabling the documentation of food used 
      by insects during their larval stages and thus increasing the accuracy and 
      precision of food web studies. The 24% success rate of our approach is 
      surprisingly high considering the challenging context for host DNA preservation, 
      and we discuss the factors possibly affecting this rate. We propose molecular 
      analysis of parasitoid linkages (MAPL) as a new method to document 
      host-parasitoid associations at a faster pace and with unrivalled precision. 
      Because of the key regulatory role of parasitoid wasps in ecosystems, which makes 
      them the most commonly used biological control agents, MAPL will have immediate 
      applications in both basic and applied biological sciences.
CI  - (c) 2010 Blackwell Publishing Ltd.
FAU - Rougerie, Rodolphe
AU  - Rougerie R
AD  - Biodiversity Institute of Ontario, University of Guelph, 50 Stone Road East, 
      Ontario, Canada. rrougeri@gmail.com
FAU - Smith, M Alex
AU  - Smith MA
FAU - Fernandez-Triana, Jose
AU  - Fernandez-Triana J
FAU - Lopez-Vaamonde, Carlos
AU  - Lopez-Vaamonde C
FAU - Ratnasingham, Sujeevan
AU  - Ratnasingham S
FAU - Hebert, Paul D N
AU  - Hebert PD
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20101118
PL  - England
TA  - Mol Ecol
JT  - Molecular ecology
JID - 9214478
SB  - IM
MH  - Animals
MH  - Food Chain
MH  - Host-Parasite Interactions
MH  - Larva/*physiology
MH  - Lepidoptera/genetics/*parasitology/physiology
MH  - Pest Control, Biological
MH  - Phylogeny
MH  - Wasps/*physiology
EDAT- 2010/11/19 06:00
MHDA- 2011/03/31 06:00
CRDT- 2010/11/19 06:00
PHST- 2010/11/19 06:00 [entrez]
PHST- 2010/11/19 06:00 [pubmed]
PHST- 2011/03/31 06:00 [medline]
AID - 10.1111/j.1365-294X.2010.04918.x [doi]
PST - ppublish
SO  - Mol Ecol. 2011 Jan;20(1):179-86. doi: 10.1111/j.1365-294X.2010.04918.x. Epub 2010 
      Nov 18.