PMID- 21084459
OWN - NLM
STAT- MEDLINE
DCOM- 20110421
LR  - 20240503
IS  - 1556-679X (Electronic)
IS  - 1556-6811 (Print)
IS  - 1556-679X (Linking)
VI  - 18
IP  - 1
DP  - 2011 Jan
TI  - Human leukocyte antigens A*3001 and A*3002 show distinct peptide-binding patterns 
      of the Mycobacterium tuberculosis protein TB10.4: consequences for immune 
      recognition.
PG  - 125-34
LID - 10.1128/CVI.00302-10 [doi]
AB  - High-tuberculosis (TB)-burden countries are located in sub-Saharan Africa. We 
      examined the frequency of human leukocyte antigen (HLA) alleles, followed by 
      recombinant expression of the most frequent HLA-A alleles, i.e., HLA-A*3001 and 
      HLA-A*3002, to study differences in mycobacterial peptide presentation and CD8(+) 
      T-cell recognition. We screened a peptide library (9-mer peptides with an 
      8-amino-acid overlap) for binding, affinity, and off-rate of the Mycobacterium 
      tuberculosis-associated antigen TB10.4 and identified only three TB10.4 peptides 
      with considerable binding to HLA-A*3001. In contrast, 22 peptides bound to 
      HLA-A*3002. This reflects a marked difference in the binding preference between 
      the two alleles, with A*3002 tolerating a more promiscuous peptide-binding 
      pattern and A*3001 accommodating only a very selective peptide repertoire. 
      Subsequent analysis of the affinity and off-rate of the binding peptides revealed 
      a strong affinity (8 nM to 7 muM) and moderate off-rate (20 min to 3 h) for both 
      alleles. Construction of HLA-A*3001 and HLA-A*3002 tetramers containing selected 
      binding peptides from TB10.4, including a peptide which was shared among both 
      alleles, QIMYNYPAM (TB10.4(3-11)), allowed us to enumerate epitope-specific T 
      cells in HLA-A*3001- and HLA-A*3002-typed patients with active TB. HLA-A*3001 and 
      HLA-A*3002 major histocompatibility complex-peptide complexes were recognized in 
      individuals with active TB, irrespective of their homozygous HLA-A*3001 or 
      HLA-A*3002 genetic background. The antigen-specific T cells exhibited the 
      CD45RA(+) CCR7(+) precursor phenotype and the interleukin-7 receptor (CD127), 
      which were different from the phenotype and receptor exhibited by the parental 
      CD8(+) T-cell population.
FAU - Axelsson-Robertson, Rebecca
AU  - Axelsson-Robertson R
AD  - Department of Microbiology and Tumor Cell Biology, Karolinska Institutet, Nobels 
      Vag 18, Stockholm SE-171 82, Sweden.
FAU - Ahmed, Raija K
AU  - Ahmed RK
FAU - Weichold, Frank F
AU  - Weichold FF
FAU - Ehlers, Marthie M
AU  - Ehlers MM
FAU - Kock, Marleen M
AU  - Kock MM
FAU - Sizemore, Donata
AU  - Sizemore D
FAU - Sadoff, Jerry
AU  - Sadoff J
FAU - Maeurer, Markus
AU  - Maeurer M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20101117
PL  - United States
TA  - Clin Vaccine Immunol
JT  - Clinical and vaccine immunology : CVI
JID - 101252125
RN  - 0 (Antigens, Bacterial)
RN  - 0 (Epitopes)
RN  - 0 (HLA Antigens)
RN  - 0 (HLA-A Antigens)
RN  - 0 (HLA-A*30:01 antigen)
RN  - 0 (Peptide Library)
RN  - 0 (Peptides)
RN  - 0 (TB10.4 antigen, Mycobacterium tuberculosis)
SB  - IM
MH  - Amino Acid Sequence
MH  - Antigens, Bacterial/*chemistry/genetics/*immunology/metabolism
MH  - Binding Sites
MH  - CD8-Positive T-Lymphocytes/immunology
MH  - Epitopes/genetics/immunology
MH  - HLA Antigens/chemistry/genetics/*immunology/metabolism
MH  - HLA-A Antigens/chemistry/genetics/*immunology/metabolism
MH  - Humans
MH  - Molecular Sequence Data
MH  - Mycobacterium tuberculosis
MH  - Peptide Library
MH  - Peptides/chemistry/*immunology/metabolism
MH  - Protein Binding
MH  - South Africa
MH  - Tuberculosis, Pulmonary/immunology
PMC - PMC3019778
EDAT- 2010/11/19 06:00
MHDA- 2011/04/22 06:00
PMCR- 2011/07/01
CRDT- 2010/11/19 06:00
PHST- 2010/11/19 06:00 [entrez]
PHST- 2010/11/19 06:00 [pubmed]
PHST- 2011/04/22 06:00 [medline]
PHST- 2011/07/01 00:00 [pmc-release]
AID - CVI.00302-10 [pii]
AID - 0302-10 [pii]
AID - 10.1128/CVI.00302-10 [doi]
PST - ppublish
SO  - Clin Vaccine Immunol. 2011 Jan;18(1):125-34. doi: 10.1128/CVI.00302-10. Epub 2010 
      Nov 17.