PMID- 21087968
OWN - NLM
STAT- MEDLINE
DCOM- 20110527
LR  - 20221207
IS  - 1552-5783 (Electronic)
IS  - 0146-0404 (Linking)
VI  - 52
IP  - 3
DP  - 2011 Mar 25
TI  - In vivo efficacy of bone marrow stromal cells coated with beta-tricalcium 
      phosphate for the reconstruction of orbital defects in canines.
PG  - 1735-41
LID - 10.1167/iovs.10-5988 [doi]
AB  - PURPOSE: To repair the segmental orbital rim defects of dogs with 
      three-dimensional (3D) tissue-engineered constructs derived from culturing 
      autogenous bone marrow stromal cells (BMSCs) on beta-tricalcium phosphate (beta-TCP) 
      scaffolds. METHODS: A 25-mm segmental defect on the canine inferior orbital rim 
      was created. BMSCs were isolated and osteogenically induced in vitro, then were 
      seeded onto 3D beta-TCP scaffolds and implanted to repair the orbital defects after 
      5 days of cultivation. The group of noninduced BMSC/beta-TCP, beta-TCP alone, and the 
      normal inferior orbital rim were set as controls. The orbits of all groups had 
      spiral computed tomography (CT) scans 1, 4, 8, and 12 weeks after surgery. Gross 
      examination, bone density, microCT, and histologic measurements were performed 12 
      weeks after surgery. The results were analyzed to evaluate the extent of bone 
      repair. RESULTS: Twelve weeks after surgery, CT examination revealed good 
      inferior orbital rim recovery in the induced BMSC/beta-TCP group, and the bone 
      density was 0.30 +/- 0.03 g/cm(2) with no dominant variance, compared with the 
      normal control (P > 0.05). MicroCT and histologic examination confirmed that the 
      implantations led to good repair of the defects. Pore-like spongy bone surrounded 
      the implants through the section plane, with some residue remaining in the 
      center. In contrast, the noninduced BMSC/beta-TCP implants were not fully repaired, 
      and nonunion was evident. The bony density for this group was 0.23 +/- 0.07 
      g/cm(2), which was significantly lower than that of the control group (P < 0.05). 
      The beta-TCP group was largely held by fibrous tissues. CONCLUSIONS: Engineered bone 
      from induced BMSCs and 3D biodegradable beta-TCP can efficiently repair 
      critical-sized segmental orbital defects in dogs.
FAU - Zhou, Huifang
AU  - Zhou H
AD  - Department of Ophthalmology, Ninth People's Hospital, Shanghai JiaoTong 
      University School of Medicine, Shanghai, P. R. China.
FAU - Xiao, Caiwen
AU  - Xiao C
FAU - Wang, Yefei
AU  - Wang Y
FAU - Bi, Xiaoping
AU  - Bi X
FAU - Ge, Shengfang
AU  - Ge S
FAU - Fan, Xianqun
AU  - Fan X
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110325
PL  - United States
TA  - Invest Ophthalmol Vis Sci
JT  - Investigative ophthalmology & visual science
JID - 7703701
RN  - 0 (Calcium Phosphates)
RN  - 0 (Coated Materials, Biocompatible)
RN  - 0 (beta-tricalcium phosphate)
SB  - IM
MH  - Absorptiometry, Photon
MH  - Animals
MH  - Bone Density
MH  - Bone Marrow Cells/*cytology/physiology
MH  - *Bone Marrow Transplantation
MH  - Calcium Phosphates/*chemistry
MH  - Cells, Cultured
MH  - Coated Materials, Biocompatible/*chemistry
MH  - Disease Models, Animal
MH  - Dogs
MH  - Orbital Fractures/diagnostic imaging/pathology/*therapy
MH  - Osteogenesis/physiology
MH  - *Plastic Surgery Procedures
MH  - Stromal Cells/*cytology/physiology
MH  - Tissue Engineering
MH  - Tissue Scaffolds
MH  - Tomography, Spiral Computed
EDAT- 2010/11/23 06:00
MHDA- 2011/05/28 06:00
CRDT- 2010/11/20 06:00
PHST- 2010/11/20 06:00 [entrez]
PHST- 2010/11/23 06:00 [pubmed]
PHST- 2011/05/28 06:00 [medline]
AID - iovs.10-5988 [pii]
AID - 10.1167/iovs.10-5988 [doi]
PST - epublish
SO  - Invest Ophthalmol Vis Sci. 2011 Mar 25;52(3):1735-41. doi: 10.1167/iovs.10-5988.