PMID- 21088148
OWN - NLM
STAT- MEDLINE
DCOM- 20101214
LR  - 20151119
IS  - 1943-7722 (Electronic)
IS  - 0002-9173 (Linking)
VI  - 134
IP  - 6
DP  - 2010 Dec
TI  - Molecular and immunohistologic analyses cannot reliably solve diagnostic 
      variation of flat intraepithelial lesions of the urinary bladder.
PG  - 862-72
LID - 10.1309/AJCPACNUDWEN9GN4 [doi]
AB  - We examined diagnostic variation of flat intraurothelial lesions with comparison 
      with immunohistochemical and fluorescence in situ hybridization (FISH) analyses. 
      Nine uropathologists diagnosed 23 biopsy samples from the urinary bladder. The 
      samples were analyzed by immunohistochemical expression of cytokeratin 20, 
      high-molecular-weight cytokeratin, Ki-67, p53, and p16(INK4a), and multicolor 
      FISH using the UroVysion probe set (Vysis, Abbott, Des Plaines, IL). Diagnostic 
      agreement for each classification and for nonneoplastic or neoplastic lesions was 
      obtained in 8 (35%) and 16 (70%) of 23 lesions, respectively. The preference 
      ratio of neoplasia to nonneoplasia (0.9 to 4.8) or carcinoma in situ to dysplasia 
      (0.2 to 4.0) also varied among the pathologists. In 6 ancillary analyses, the 
      majority of neoplastic lesions with diagnostic agreement indicated more than 2 
      aberrant results, whereas the majority of lesions without diagnostic agreement 
      showed no or only 1 aberrant result. The molecular and immunohistochemical 
      analyses can discriminate between neoplastic and nonneoplastic lesions; however, 
      they cannot reliably solve diagnostic variation of flat intraepithelial lesions.
FAU - Murata, Shin-ichi
AU  - Murata S
AD  - Department of Pathology, Saitama Medical University International Medical Center, 
      1397-1 Yamane, Hidaka-shi, Saitama-ken, 350-1298, Japan.
FAU - Iseki, Masachika
AU  - Iseki M
FAU - Kinjo, Mitsuru
AU  - Kinjo M
FAU - Matsuzaki, Osamu
AU  - Matsuzaki O
FAU - Moriuchi, Akira
AU  - Moriuchi A
FAU - Ohtani, Hiroshi
AU  - Ohtani H
FAU - Sakurai, Takaki
AU  - Sakurai T
FAU - Satake, Tatsunari
AU  - Satake T
FAU - Tsuzuki, Toyonori
AU  - Tsuzuki T
LA  - eng
PT  - Journal Article
PL  - England
TA  - Am J Clin Pathol
JT  - American journal of clinical pathology
JID - 0370470
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (Cyclin-Dependent Kinase Inhibitor p16)
RN  - 0 (DNA, Neoplasm)
RN  - 0 (Keratin-20)
RN  - 0 (Ki-67 Antigen)
RN  - 0 (Tumor Suppressor Protein p53)
SB  - IM
MH  - Aged
MH  - Aged, 80 and over
MH  - Biomarkers, Tumor/genetics/metabolism
MH  - Biopsy
MH  - Carcinoma in Situ/*diagnosis/genetics/metabolism
MH  - Carcinoma, Papillary/*diagnosis/genetics/metabolism
MH  - Carcinoma, Transitional Cell/*diagnosis/genetics/metabolism
MH  - Cyclin-Dependent Kinase Inhibitor p16/metabolism
MH  - DNA, Neoplasm/genetics
MH  - Female
MH  - Humans
MH  - Immunohistochemistry/methods
MH  - In Situ Hybridization, Fluorescence/methods
MH  - Keratin-20/metabolism
MH  - Ki-67 Antigen/metabolism
MH  - Male
MH  - Middle Aged
MH  - Observer Variation
MH  - Precancerous Conditions/*diagnosis/genetics/metabolism
MH  - Reproducibility of Results
MH  - Tumor Suppressor Protein p53/metabolism
MH  - Urinary Bladder Neoplasms/*diagnosis/genetics/metabolism
MH  - Urothelium/metabolism/*pathology
EDAT- 2010/11/23 06:00
MHDA- 2010/12/16 06:00
CRDT- 2010/11/20 06:00
PHST- 2010/11/20 06:00 [entrez]
PHST- 2010/11/23 06:00 [pubmed]
PHST- 2010/12/16 06:00 [medline]
AID - 134/6/862 [pii]
AID - 10.1309/AJCPACNUDWEN9GN4 [doi]
PST - ppublish
SO  - Am J Clin Pathol. 2010 Dec;134(6):862-72. doi: 10.1309/AJCPACNUDWEN9GN4.