PMID- 2108896
OWN - NLM
STAT- MEDLINE
DCOM- 19900518
LR  - 20211216
IS  - 0378-1097 (Print)
IS  - 0378-1097 (Linking)
VI  - 54
IP  - 1-3
DP  - 1990 Jan 1
TI  - Differences between the mechanisms of action of heat-stable and heat-labile 
      enterotoxins of Escherichia coli.
PG  - 169-73
AB  - The mucosal-to-serosal and serosal-to-mucosal fluxes of Na+ and Cl- were measured 
      in control mice and mice treated with heat-stable (ST) and heat-labile (LT) 
      enterotoxins in the presence or absence of: Ca2(+)-ionophore A23187, an activator 
      of Ca2(+)-calmodulin; or phorbol-12-myristate-13-acetate (PMA), an activator of 
      protein kinase C(PKC); or 1-(5-isoquinolinyl sulphonyl)-2-methyl piperazine 
      (H-7), an inhibitor of PKC. There was net secretion of Na+ and CL- in both 
      experimental groups in contrast to net absorption in the control group. The 
      addition of ionophore or PMA or ionophore + PMA resulted in net secretion of Na+ 
      and Cl- in the control group and the effect of ionophore and pMA was found to be 
      additive. The addition of ionophore did not cause any change in electrolyte 
      fluxes in the ST toxin treated group, however, it increased the net secretion of 
      Na+ and Cl- in the LT toxin treated group. PMA increased the net secretion of Na+ 
      and Cl- in the St toxin treated group, however, it did not cause any change in 
      Na+ and Cl- fluxes in the LT toxin treated group. H-7 did not reverse the effect 
      of ST toxin, however, it reversed the effect of LT toxin.(ABSTRACT TRUNCATED AT 
      250 WORDS)
FAU - Goyal, J
AU  - Goyal J
AD  - Department of Paediatrics, Postgraduate Institute of Medical Education and 
      Research, Chandigarh, India.
FAU - Ganguly, N K
AU  - Ganguly NK
FAU - Garg, U C
AU  - Garg UC
FAU - Walia, B N
AU  - Walia BN
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - England
TA  - FEMS Microbiol Lett
JT  - FEMS microbiology letters
JID - 7705721
RN  - 0 (Bacterial Toxins)
RN  - 0 (Calmodulin)
RN  - 0 (Chlorides)
RN  - 0 (Enterotoxins)
RN  - 0 (Escherichia coli Proteins)
RN  - 0 (Isoquinolines)
RN  - 0 (Piperazines)
RN  - 0 (heat stable toxin (E coli))
RN  - 37H9VM9WZL (Calcimycin)
RN  - 84477-87-2 (1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine)
RN  - 9NEZ333N27 (Sodium)
RN  - D9K3SN2LNY (heat-labile enterotoxin, E coli)
RN  - EC 2.7.11.13 (Protein Kinase C)
RN  - NI40JAQ945 (Tetradecanoylphorbol Acetate)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - 1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine
MH  - Animals
MH  - Bacterial Toxins/*pharmacology
MH  - Biological Transport, Active/drug effects
MH  - Calcimycin/pharmacology
MH  - Calcium/metabolism
MH  - Calmodulin/metabolism
MH  - Chlorides/metabolism
MH  - Enterotoxins/*pharmacology
MH  - Escherichia coli
MH  - *Escherichia coli Proteins
MH  - Ileum/*drug effects/metabolism
MH  - Isoquinolines/pharmacology
MH  - Mice
MH  - Piperazines/pharmacology
MH  - Protein Kinase C/metabolism
MH  - Sodium/metabolism
MH  - Tetradecanoylphorbol Acetate/pharmacology
EDAT- 1990/01/01 00:00
MHDA- 1990/01/01 00:01
CRDT- 1990/01/01 00:00
PHST- 1990/01/01 00:00 [pubmed]
PHST- 1990/01/01 00:01 [medline]
PHST- 1990/01/01 00:00 [entrez]
AID - 10.1016/0378-1097(90)90277-w [doi]
PST - ppublish
SO  - FEMS Microbiol Lett. 1990 Jan 1;54(1-3):169-73. doi: 
      10.1016/0378-1097(90)90277-w.