PMID- 21089171 OWN - NLM STAT- MEDLINE DCOM- 20110315 LR - 20220310 IS - 1098-2825 (Electronic) IS - 0887-8013 (Print) IS - 0887-8013 (Linking) VI - 24 IP - 6 DP - 2010 TI - Preliminary evaluation of Hitchens-Pike-Todd-Hewitt medium (HPTH) for detection of Group B Streptococci in pregnant women. PG - 403-6 LID - 10.1002/jcla.20419 [doi] AB - We aimed to compare Hitchens-Pike-Todd-Hewitt (HPTH) enrichment broth, Todd-Hewitt broth, and direct culture onto blood sheep agar containing selective antimicrobial (ABA) in screening Group B Streptococci (GBS) carriage in pregnant women. From April to June 2007, duplicate lower vaginal and duplicate anorectal specimens of 102 pregnant women at 35 weeks of gestation or more, who were assisted at Santa Casa de Misericordia Hospital, Maringa, Parana, Brazil, were screened by GBS using HPTH, Todd-Hewitt broth, and ABA. Twenty-five (24.5%) pregnant women had one or more positive culture for GBS in those media. The positive rate for each medium was 21.6% (22/102) in the HPTH, 12.8% (13/102) in ABA, and 11.8% (12/102) in Todd-Hewitt broth. HPTH seems to be a high satisfactory medium for screening GBS in vaginal and anorectal samples in pregnant women and additional studies would be interesting in a larger number of pregnant women and in different laboratories. CI - (c) 2010 Wiley-Liss, Inc. FAU - Chaves, Mauricio Jr AU - Chaves M Jr AD - Department of Medicine, State University of Maringa, Parana, Brazil. FAU - Padua, Rubia A F AU - Padua RA FAU - Campanerut, Paula A Z AU - Campanerut PA FAU - Pelloso, Sandra M AU - Pelloso SM FAU - Carvalho, Maria Dalva B AU - Carvalho MD FAU - Siqueira, Vera L D AU - Siqueira VL FAU - Scodro, Regiane B L AU - Scodro RB FAU - Cardoso, Rosilene F AU - Cardoso RF LA - eng PT - Comparative Study PT - Journal Article PL - United States TA - J Clin Lab Anal JT - Journal of clinical laboratory analysis JID - 8801384 RN - 0 (Culture Media) SB - IM MH - Anal Canal/microbiology MH - Brazil/epidemiology MH - Carrier State/*diagnosis/epidemiology/microbiology MH - *Culture Media MH - Female MH - Humans MH - Mass Screening/methods MH - Pregnancy MH - Pregnancy Complications, Infectious/*diagnosis/epidemiology/microbiology MH - Pregnancy Trimester, Third MH - Prevalence MH - Rectum/microbiology MH - Streptococcal Infections/*diagnosis/epidemiology/microbiology MH - Streptococcus/growth & development/*isolation & purification MH - Vagina/microbiology PMC - PMC6647687 EDAT- 2010/11/23 06:00 MHDA- 2011/03/16 06:00 PMCR- 2010/11/18 CRDT- 2010/11/20 06:00 PHST- 2010/11/20 06:00 [entrez] PHST- 2010/11/23 06:00 [pubmed] PHST- 2011/03/16 06:00 [medline] PHST- 2010/11/18 00:00 [pmc-release] AID - JCLA20419 [pii] AID - 10.1002/jcla.20419 [doi] PST - ppublish SO - J Clin Lab Anal. 2010;24(6):403-6. doi: 10.1002/jcla.20419.