PMID- 2108948
OWN - NLM
STAT- MEDLINE
DCOM- 19900521
LR  - 20190510
IS  - 0910-5050 (Print)
IS  - 1876-4673 (Electronic)
IS  - 0910-5050 (Linking)
VI  - 81
IP  - 1
DP  - 1990 Jan
TI  - Identification of a protein increasing in serum of Nagase analbuminemic rats 
      bearing intestinal tumors as an isotype of T-kininogen.
PG  - 63-8
AB  - Increase in an unidentified protein was observed in serum of Nagase analbuminemic 
      rats (NAR) bearing intestinal tumors induced by azoxymethane. This protein seemed 
      to be a polymer of a protein of 73 kDa as estimated by sodium dodecyl 
      sulfate-polyacrylamide gel electrophoresis, and so was tentatively named 
      73K-protein. The serum concentration of 73K-protein in NAR bearing intestinal 
      tumors was 11.9 +/- 2.2 mg/ml (mean +/- SD, n = 5), whereas that in control NAR 
      was 2.0 +/- 0.2 mg/ml. Increase of the serum 73K-protein level was also observed 
      in Sprague-Dawley rats bearing intestinal tumors, skin tumors, subcutaneous 
      sarcomas, or mammary tumors and in ACI rats bearing urinary bladder tumors. On 
      double immunodiffusion analysis, the 73K-protein was not detected in mouse, 
      guinea pig, pig, horse, or human serum. A cDNA clone bearing the sequence 
      encoding 73K-protein was isolated from a cDNA library constructed from rat liver 
      mRNA. The nucleotide sequence of the 73K-protein showed 98.8% and 96.9% 
      homologies with the sequences of the 3'-proximal domains of the cDNAs for TI- and 
      TII-kininogen, respectively. Therefore, the 73K-protein was concluded to be an 
      isotype of T-kininogen.
FAU - Kanda, S
AU  - Kanda S
AD  - Department of Chemistry, Sasaki Institute, Tokyo.
FAU - Sugiyama, K
AU  - Sugiyama K
FAU - Takahashi, M
AU  - Takahashi M
FAU - Shumiya, S
AU  - Shumiya S
FAU - Tomino, S
AU  - Tomino S
FAU - Nagase, S
AU  - Nagase S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Japan
TA  - Jpn J Cancer Res
JT  - Japanese journal of cancer research : Gann
JID - 8509412
RN  - 0 (Blood Proteins)
RN  - 0 (Kininogens)
RN  - 0 (Serum Albumin)
RN  - 9007-49-2 (DNA)
RN  - MO0N1J0SEN (Azoxymethane)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Azoxymethane
MH  - Base Sequence
MH  - Blood Proteins/*metabolism
MH  - Cloning, Molecular
MH  - DNA/genetics
MH  - Immunodiffusion
MH  - Intestinal Neoplasms/*blood/chemically induced/complications
MH  - Kininogens/*blood
MH  - Molecular Sequence Data
MH  - Molecular Weight
MH  - Rats
MH  - Rats, Inbred Strains
MH  - Rats, Mutant Strains
MH  - Serum Albumin/*deficiency
PMC - PMC5917951
EDAT- 1990/01/01 00:00
MHDA- 1990/01/01 00:01
PMCR- 1990/01/01
CRDT- 1990/01/01 00:00
PHST- 1990/01/01 00:00 [pubmed]
PHST- 1990/01/01 00:01 [medline]
PHST- 1990/01/01 00:00 [entrez]
PHST- 1990/01/01 00:00 [pmc-release]
AID - CAE63 [pii]
AID - 10.1111/j.1349-7006.1990.tb02508.x [doi]
PST - ppublish
SO  - Jpn J Cancer Res. 1990 Jan;81(1):63-8. doi: 10.1111/j.1349-7006.1990.tb02508.x.