PMID- 2109025
OWN - NLM
STAT- MEDLINE
DCOM- 19900521
LR  - 20081121
IS  - 0737-1454 (Print)
IS  - 0737-1454 (Linking)
VI  - 8 Suppl 1
DP  - 1990 Jan
TI  - Regulation of cellular gene expression by interferon-gamma: involvement of 
      multiple pathways.
PG  - 92-102
AB  - Interferons (IFNs) classified as type I (IFN-alpha and -beta) and type II 
      (IFN-gamma) interact with different receptors and regulate the expression of a 
      number of genes in common, whereas the expression of certain other genes is 
      regulated differentially by the type I or type II IFNs. Regulation of cellular 
      gene expression by IFN-gamma was studied with the help of two cDNA clones (called 
      C5-4 and C13) isolated in our laboratory and cDNA clones for human leukocyte 
      antigen (HLA) class I (HLA-B) and class II (HLA-DR alpha, -DR beta) genes. The 
      results indicate that IFN-gamma induced the expression of the cognate genes, but 
      in different manners. IFN-gamma induced the transcription of all four genes as 
      determined by nuclear run-on transcription analyses, but the induction of C5-4 
      genes transcription was inhibited by cycloheximide and anisomycin, indicating 
      that some newly synthesized protein, presumably induced by IFN-gamma, was 
      required for the transcriptional activation of the C5-4 gene. On the contrary, 
      IFN-gamma-induced transcription of HLA class I, class II and C13 genes was 
      unaffected by cycloheximide or anisomycin. However, these inhibitors completely 
      blocked the accumulation of the HLA class II gene transcripts, but not HLA class 
      I or C13 gene transcripts. Results suggest that some newly synthesized protein 
      factor was required for IFN-gamma-induced accumulation of HLA class II gene 
      transcripts and played a role at a step subsequent to the transcriptional 
      activation by IFN-gamma. Evidence was obtained which suggests that the putative 
      protein factor(s) required was induced by IFN-gamma. These studies indicate that 
      IFN-gamma regulates the expression of cellular genes through multiple pathways.
FAU - Gupta, S L
AU  - Gupta SL
AD  - Hipple Cancer Research Center, Dayton, Ohio 45439.
LA  - eng
GR  - CA-29991/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PT  - Review
PL  - United States
TA  - Int J Cell Cloning
JT  - International journal of cell cloning
JID - 8308172
RN  - 0 (RNA, Messenger)
RN  - 82115-62-6 (Interferon-gamma)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Cloning, Molecular
MH  - DNA/isolation & purification
MH  - Gene Expression Regulation/*drug effects
MH  - Humans
MH  - Interferon-gamma/*pharmacology
MH  - RNA, Messenger/biosynthesis/drug effects
MH  - Signal Transduction
RF  - 21
EDAT- 1990/01/01 00:00
MHDA- 1990/01/01 00:01
CRDT- 1990/01/01 00:00
PHST- 1990/01/01 00:00 [pubmed]
PHST- 1990/01/01 00:01 [medline]
PHST- 1990/01/01 00:00 [entrez]
AID - 10.1002/stem.5530080709 [doi]
PST - ppublish
SO  - Int J Cell Cloning. 1990 Jan;8 Suppl 1:92-102. doi: 10.1002/stem.5530080709.