PMID- 21091668
OWN - NLM
STAT- MEDLINE
DCOM- 20110203
LR  - 20211020
IS  - 1365-2249 (Electronic)
IS  - 0009-9104 (Print)
IS  - 0009-9104 (Linking)
VI  - 163
IP  - 2
DP  - 2011 Feb
TI  - Toll-like receptor (TLR)-4 mediates anti-beta2GPI/beta2GPI-induced tissue factor 
      expression in THP-1 cells.
PG  - 189-98
LID - 10.1111/j.1365-2249.2010.04291.x [doi]
AB  - Our previous study demonstrated that annexin A2 (ANX2) on cell surface could 
      function as a mediator and stimulate tissue factor (TF) expression of monocytes 
      by anti-beta(2)-glycoprotein I/beta(2)-glycoprotein I complex (anti-beta(2)GPI/beta(2)GPI). However, 
      ANX2 is not a transmembrane protein and lacks the intracellular signal 
      transduction pathway. Growing evidence suggests that Toll-like receptor 4 (TLR-4) 
      might act as an 'adaptor' for intracellular signal transduction in 
      anti-beta(2)GPI/beta(2)GPI-induced TF expressing cells. In the current study, we 
      investigated the roles of TLR-4 and its related molecules, myeloid 
      differentiation protein 2 (MD-2) and myeloid differentiation factor 88 (MyD88), 
      in anti-beta(2)GPI/beta(2)GPI-induced TF expressing human monocytic-derived THP-1 (human 
      acute monocytic leukaemia) cells. The relationship of TLR-4 and ANX2 in this 
      process was also explored. Along with TF, expression of TLR-4, MD-2 and MyD88 in 
      THP-1 cells increased significantly when treated by anti-beta(2)GPI (10 microg/ml)/beta(2)GPI 
      (100 microg/ml) complex. The addition of paclitaxel, which competes with the MD-2 
      ligand, could inhibit the effects of anti-beta(2)GPI/beta(2)GPI on TLR-4, MD-2, MyD88 and 
      TF expression. Both ANX2 and TLR-4 in THP-1 cell lysates could bind to beta(2)GPI that 
      had been conjugated to a column (beta(2)GPI-Affi-Gel). Furthermore, TLR-4, MD-2, MyD88 
      and TF expression was remarkably diminished in THP-1 cells infected with 
      ANX2-specific RNA interference (RNAi) lentivirus (LV-RNAi-ANX2), in spite of 
      treatment with a similar concentration of anti-beta(2)GPI/beta(2)GPI complex. These results 
      indicate that TLR-4 and its signal transduction pathway contribute to 
      anti-beta(2)GPI/beta(2)GPI-induced TF expression in THP-1 cells, and the effects of TLR-4 
      with ANX2 are tightly co-operative.
CI  - (c) 2010 The Authors. Clinical and Experimental Immunology (c) 2010 British Society 
      for Immunology.
FAU - Zhou, H
AU  - Zhou H
AD  - Department of Clinical Laboratory and Hematology, Jiangsu University, Zhenjiang, 
      China. hongzhou@ujs.edu.cn
FAU - Yan, Y
AU  - Yan Y
FAU - Xu, G
AU  - Xu G
FAU - Zhou, B
AU  - Zhou B
FAU - Wen, H
AU  - Wen H
FAU - Guo, D
AU  - Guo D
FAU - Zhou, F
AU  - Zhou F
FAU - Wang, H
AU  - Wang H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20101122
PL  - England
TA  - Clin Exp Immunol
JT  - Clinical and experimental immunology
JID - 0057202
RN  - 0 (Annexin A2)
RN  - 0 (Antibodies, Antiphospholipid)
RN  - 0 (LY96 protein, human)
RN  - 0 (Lymphocyte Antigen 96)
RN  - 0 (Myeloid Differentiation Factor 88)
RN  - 0 (TLR4 protein, human)
RN  - 0 (Toll-Like Receptor 4)
RN  - 0 (beta 2-Glycoprotein I)
RN  - 9035-58-9 (Thromboplastin)
RN  - P88XT4IS4D (Paclitaxel)
SB  - IM
MH  - Annexin A2/metabolism
MH  - Antibodies, Antiphospholipid/*immunology
MH  - Antiphospholipid Syndrome/*immunology
MH  - Cell Line
MH  - Humans
MH  - Lymphocyte Antigen 96/metabolism
MH  - Monocytes/drug effects/immunology
MH  - Myeloid Differentiation Factor 88/metabolism
MH  - Paclitaxel/pharmacology
MH  - Thromboplastin/*biosynthesis
MH  - Toll-Like Receptor 4/*metabolism
MH  - beta 2-Glycoprotein I/*antagonists & inhibitors/immunology
PMC - PMC3043309
EDAT- 2010/11/26 06:00
MHDA- 2011/02/04 06:00
PMCR- 2012/02/01
CRDT- 2010/11/25 06:00
PHST- 2010/11/25 06:00 [entrez]
PHST- 2010/11/26 06:00 [pubmed]
PHST- 2011/02/04 06:00 [medline]
PHST- 2012/02/01 00:00 [pmc-release]
AID - 10.1111/j.1365-2249.2010.04291.x [doi]
PST - ppublish
SO  - Clin Exp Immunol. 2011 Feb;163(2):189-98. doi: 10.1111/j.1365-2249.2010.04291.x. 
      Epub 2010 Nov 22.