PMID- 21091725
OWN - NLM
STAT- MEDLINE
DCOM- 20110216
LR  - 20200930
IS  - 1365-2761 (Electronic)
IS  - 0140-7775 (Linking)
VI  - 33
IP  - 12
DP  - 2010 Dec
TI  - Comparative analysis of the phenotypic characteristics of high- and low-virulent 
      strains of Edwardsiella tarda.
PG  - 985-94
LID - 10.1111/j.1365-2761.2010.01204.x [doi]
AB  - Edwardsiella tarda is a causative agent of edwardsiellosis in freshwater and 
      marine fish. Extracellular enzymic, haemolytic, hydrophobic and serum resistance 
      activities, haemagglutination, autoagglutination and siderophores of high- and 
      low- virulent E. tarda strains were examined. The results revealed different 
      haemagglutination, autoagglutination, haemolytic, hydrophobic and serum 
      resistance activities in different strains. Analysis of extracellular proteins 
      (ECPs) and outer membrane proteins (OMPs) demonstrated several major, low 
      molecular weight, virulent-strain-specific proteins, which could be 
      virulence-related. Based on the database search with MALDI-TOF MS data, the 
      closest homologies of the three protein bands Ed1, Ed2 and Ed3 were 
      phosphotransferase enzyme family protein, nitrite reductase [NAD(P)H], large 
      subunit and ATP-dependent Lon protease, respectively. A comparison of 
      pathogenicity of purified lipopolysaccharide (LPS) and lipid A from virulent and 
      avirulent strains demonstrated that LPS was one of the virulence factors of the 
      E. tarda isolates, and lipid A was a biologically active determinant of LPS.
CI  - (c) 2010 Blackwell Publishing Ltd.
FAU - Wang, Y
AU  - Wang Y
AD  - Department of Marine Biology, Ocean University of China, Qingdao, China.
FAU - Zhang, X H
AU  - Zhang XH
FAU - Austin, B
AU  - Austin B
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Fish Dis
JT  - Journal of fish diseases
JID - 9881188
RN  - 0 (Lipid A)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Virulence Factors)
SB  - IM
MH  - Animals
MH  - Edwardsiella tarda/enzymology/*pathogenicity
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Enterobacteriaceae Infections/microbiology/*veterinary
MH  - Fish Diseases/*microbiology
MH  - Lipid A/pharmacology
MH  - Lipopolysaccharides/pharmacology
MH  - Oncorhynchus mykiss
MH  - *Phenotype
MH  - Virulence Factors/*chemistry/isolation & purification/metabolism
EDAT- 2010/11/26 06:00
MHDA- 2011/02/17 06:00
CRDT- 2010/11/25 06:00
PHST- 2010/11/25 06:00 [entrez]
PHST- 2010/11/26 06:00 [pubmed]
PHST- 2011/02/17 06:00 [medline]
AID - 10.1111/j.1365-2761.2010.01204.x [doi]
PST - ppublish
SO  - J Fish Dis. 2010 Dec;33(12):985-94. doi: 10.1111/j.1365-2761.2010.01204.x.