PMID- 21091796
OWN - NLM
STAT- MEDLINE
DCOM- 20110310
LR  - 20171116
IS  - 1442-2042 (Electronic)
IS  - 0919-8172 (Linking)
VI  - 17
IP  - 12
DP  - 2010 Dec
TI  - Calcium oxalate crystal deposition in metabolic syndrome model rat kidneys.
PG  - 996-1003
LID - 10.1111/j.1442-2042.2010.02661.x [doi]
AB  - OBJECTIVE: Although an epidemiological link between the metabolic syndrome and 
      kidney stone formation has been reported, the mechanism by which metabolic 
      syndrome promotes kidney stone formation has yet to be elucidated. We 
      investigated calcium oxalate (CaOx) kidney stone formation in a rat metabolic 
      syndrome model. METHODS: We induced hyperoxaluria in 8-week-old male Otsuka 
      Long-Evans Tokushima fatty (OLETF) rats, and a control strain, Long-Evans 
      Tokushima Otsuka (LETO) rats, by administering 1.0% ethylene glycol (EG) as their 
      drinking water for 2 weeks. Rats were divided into four groups: LETO-C (control, 
      n = 7); LETO-SF (stone forming, n = 8); OLETF-C (n = 7); and OLETF-SF (n = 8). 
      Urine and blood samples were collected for biochemistry testing, and the kidneys 
      were harvested for estimation of crystal deposition and determinations of the 
      expression of osteopontin (OPN) and monocyte chemoattractant protein-1 (MCP-1). 
      RESULTS: Administration of EG induced hyperoxaluria to the same degree in both 
      strains. The OLETF-SF group showed a higher grade of renal crystal deposition and 
      significantly higher renal calcium content than the LETO-SF group. Although the 
      OLETF-C group excreted significantly higher amounts of uric acid and more acidic 
      urine than the LETO-C group, similar differences were not observed in rats given 
      EG. Significant upregulation of both OPN and MCP-1 was seen in the kidneys of 
      hyperoxaluric rats, with higher levels of expression in the OLETF-SF group than 
      the LETO-SF group. CONCLUSIONS: The present results show for the first time that 
      OLETF rats form more renal CaOx crystal deposits compared with control rats under 
      EG-induced hyperoxaluric conditions. The model described here should be useful 
      for investigating the mechanisms by which the metabolic syndrome promotes CaOx 
      kidney stone formation.
CI  - (c) 2010 The Japanese Urological Association.
FAU - Okamoto, Masanori
AU  - Okamoto M
AD  - Division of Nephrology and Blood Purification Medicine Department of Urology, 
      Wakayama Medical University, Wakayama, Japan. mokamoto@wakayama-med.ac.jp
FAU - Kohjimoto, Yasuo
AU  - Kohjimoto Y
FAU - Iba, Akinori
AU  - Iba A
FAU - Saji, Fumie
AU  - Saji F
FAU - Hara, Isao
AU  - Hara I
FAU - Shigematsu, Takashi
AU  - Shigematsu T
LA  - eng
PT  - Journal Article
PL  - Australia
TA  - Int J Urol
JT  - International journal of urology : official journal of the Japanese Urological 
      Association
JID - 9440237
RN  - 0 (Ccl2 protein, rat)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Spp1 protein, rat)
RN  - 106441-73-0 (Osteopontin)
RN  - 2612HC57YE (Calcium Oxalate)
SB  - IM
MH  - Animals
MH  - Body Weight/physiology
MH  - Calcium Oxalate/*chemistry/*metabolism
MH  - Chemokine CCL2/metabolism
MH  - Crystallization
MH  - Disease Models, Animal
MH  - Hyperoxaluria/metabolism/pathology
MH  - Kidney/*metabolism/pathology
MH  - Male
MH  - Metabolic Syndrome/*metabolism
MH  - Nephrolithiasis/*metabolism/pathology
MH  - Osteopontin/metabolism
MH  - Rats
MH  - Rats, Inbred OLETF
MH  - Rats, Long-Evans
MH  - Urine/chemistry
EDAT- 2010/11/26 06:00
MHDA- 2011/03/11 06:00
CRDT- 2010/11/25 06:00
PHST- 2010/11/25 06:00 [entrez]
PHST- 2010/11/26 06:00 [pubmed]
PHST- 2011/03/11 06:00 [medline]
AID - 10.1111/j.1442-2042.2010.02661.x [doi]
PST - ppublish
SO  - Int J Urol. 2010 Dec;17(12):996-1003. doi: 10.1111/j.1442-2042.2010.02661.x.