PMID- 21092010
OWN - NLM
STAT- MEDLINE
DCOM- 20110502
LR  - 20161018
IS  - 1939-1676 (Electronic)
IS  - 0891-6640 (Linking)
VI  - 25
IP  - 1
DP  - 2011 Jan-Feb
TI  - Use of serum concentrations of interleukin-18 and monocyte chemoattractant 
      protein-1 as prognostic indicators in primary immune-mediated hemolytic anemia in 
      dogs.
PG  - 76-82
LID - 10.1111/j.1939-1676.2010.0642.x [doi]
AB  - BACKGROUND: The cytokine response in immune-mediated hemolytic anemia (IMHA) is 
      poorly characterized and correlation with outcome is unknown. 
      HYPOTHESIS/OBJECTIVES: To determine if cytokine activity is correlated with 
      outcome in dogs with IMHA. ANIMALS: Twenty dogs with primary IMHA and 6 control 
      dogs. METHODS: Prospective study on dogs with IMHA with blood sampling at 
      admission. Serum activity of interleukin-2 (IL-2), IL-4, IL-6, IL-7, IL-8, IL-10, 
      IL-15, IL-18, monocyte chemoattractant protein-1 (MCP-1), granulocyte-macrophage 
      colony stimulating factor (GM-CSF), interferon-inducible protein-10, 
      interferon-gamma, and keratinocyte chemoattractant (KC) was assessed. RESULTS: 
      Thirty-day case fatality rate was 25% (5/20 dogs). Increased concentrations 
      (median [range]) of IL-2 (45.5 ng/L [0;830] versus 0 ng/L [0;46.8]), IL-10 (8.2 
      ng/L [0;60.6] versus 0 ng/L [0;88.2]), KC (1.7 mug/L [0.3;4.7] versus 0.5 mug/L 
      [0.2;1.1]), and MCP-1 (162 ng/L [97.6;438] versus 124 ng/L [90.2;168]) were 
      observed in dogs with IMHA compared with controls. The cytokine profile was 
      indicative of a mixture of pro- and anti-inflammatory cytokines of various 
      cellular origins. Cytokines/chemokines strongly associated with 
      macrophage/monocyte activation and recruitment were significantly increased in 
      nonsurvivors compared with survivors; IL-15 (179 ng/L [48.0;570] versus 21.3 ng/L 
      [0;193]), IL-18 (199 ng/L [58.7;915] versus 37.4 ng/L [0;128]), GM-CSF (134 ng/L 
      [70.0;863] versus 57.6 ng/L [0;164]), and MCP-1 (219 ng/L [135;438] versus 159 
      ng/L [97.6;274]), respectively. Logistic regression suggested increased IL-18 and 
      MCP-1 concentrations were independently associated with mortality in this 
      population (P<.05, Wald's type 3). CONCLUSIONS AND CLINICAL IMPORTANCE: A mixed 
      cytokine response is present in dogs with IMHA and mediators of macrophage 
      activation and recruitment might serve as prognostic indicators.
CI  - Copyright (c) 2010 by the American College of Veterinary Internal Medicine.
FAU - Kjelgaard-Hansen, M
AU  - Kjelgaard-Hansen M
AD  - Department of Small Animal Clinical Sciences, Faculty of Life Sciences, 
      University of Copenhagen, Frederiksberg C, Denmark. mjkh@life.ku.dk
FAU - Goggs, R
AU  - Goggs R
FAU - Wiinberg, B
AU  - Wiinberg B
FAU - Chan, D L
AU  - Chan DL
LA  - eng
PT  - Journal Article
DEP - 20101123
PL  - United States
TA  - J Vet Intern Med
JT  - Journal of veterinary internal medicine
JID - 8708660
RN  - 0 (Chemokine CCL2)
RN  - 0 (Interleukin-18)
SB  - IM
MH  - Anemia, Hemolytic, Autoimmune/blood/*veterinary
MH  - Animals
MH  - Chemokine CCL2/*blood
MH  - Dog Diseases/*blood
MH  - Dogs
MH  - Interleukin-18/*blood
MH  - Logistic Models
MH  - Predictive Value of Tests
MH  - Prospective Studies
EDAT- 2010/11/26 06:00
MHDA- 2011/05/03 06:00
CRDT- 2010/11/25 06:00
PHST- 2010/11/25 06:00 [entrez]
PHST- 2010/11/26 06:00 [pubmed]
PHST- 2011/05/03 06:00 [medline]
AID - 10.1111/j.1939-1676.2010.0642.x [doi]
PST - ppublish
SO  - J Vet Intern Med. 2011 Jan-Feb;25(1):76-82. doi: 10.1111/j.1939-1676.2010.0642.x. 
      Epub 2010 Nov 23.