PMID- 21092070
OWN - NLM
STAT- MEDLINE
DCOM- 20111118
LR  - 20220330
IS  - 1478-3231 (Electronic)
IS  - 1478-3223 (Linking)
VI  - 31
IP  - 7
DP  - 2011 Aug
TI  - Transplantation of bone marrow cells reduces CCl4 -induced liver fibrosis in 
      mice.
PG  - 932-9
LID - 10.1111/j.1478-3231.2010.02364.x [doi]
AB  - BACKGROUND: We investigated the reversibility of liver fibrosis induced with a 
      CCl(4) injection and the role of stem cells in reversing the hepatic injury. 
      Furthermore, the most effective cell fraction among bone marrow cells (BMCs) in 
      the repair process was analysed. METHODS: C57BL/6 mice were divided into four 
      groups after 5 weeks of injection of CCl(4) : control, sacrificed after 5 weeks, 
      sacrificed at 10 weeks and sacrificed 5 weeks later after GFP-donor BM 
      transplantation. Liver function tests and real-time polymerase chain reaction 
      (PCR) of markers indicating liver fibrosis were compared between the groups. To 
      identify the most effective BMC fraction that repairs liver injury, the mice were 
      divided into three groups after the injection of CCl(4) for 2 days: granulocyte 
      colony stimulating factor (G-CSF) only, mononuclear cell (MNC) transplantation 
      and Lin-Sca-1+c-kit+haematopoietic stem cell (HSC) transplantation. Eight days 
      after transplantation, the mice were harvested and morphometric, 
      immunohistochemical analyses were performed to compare the expression of 
      extracellular matrix and liver fibrosis-related factors. RESULTS: The liver 
      fibrosis induced by CCl(4) was not spontaneously recovered but was persistent 
      until 10 weeks, but the group injected with BMCs had less fibrosis and better 
      liver function. Mobilization with G-CSF increased the recovery of the injured 
      liver and the best results were seen in those mice administered the MNC fraction 
      and Lin-Sca-1+c-kit+HSC fraction, with no difference between the two groups. 
      CONCLUSION: BMC transplantation and stem cell mobilization with G-CSF effectively 
      treats liver injury in mice. These are promising techniques for autologous 
      transplantation in humans with liver fibrosis.
CI  - (c) 2010 John Wiley & Sons A/S.
FAU - Cho, Kyung-Ah
AU  - Cho KA
AD  - Department of Microbiology, School of Medicine, Ewha Womans University, Seoul, 
      Korea.
FAU - Lim, Goh-Woon
AU  - Lim GW
FAU - Joo, Sun-Young
AU  - Joo SY
FAU - Woo, So-Youn
AU  - Woo SY
FAU - Seoh, Ju-Young
AU  - Seoh JY
FAU - Cho, Su Jin
AU  - Cho SJ
FAU - Han, Ho-Seong
AU  - Han HS
FAU - Ryu, Kyung-Ha
AU  - Ryu KH
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20101124
PL  - United States
TA  - Liver Int
JT  - Liver international : official journal of the International Association for the 
      Study of the Liver
JID - 101160857
RN  - 0 (Chemokine CCL4)
RN  - 0 (DNA Primers)
RN  - 143011-72-7 (Granulocyte Colony-Stimulating Factor)
SB  - IM
MH  - Animals
MH  - Apoptosis
MH  - Bone Marrow Transplantation/*methods
MH  - Chemokine CCL4/toxicity
MH  - DNA Primers/genetics
MH  - Granulocyte Colony-Stimulating Factor/administration & dosage
MH  - Hematopoietic Stem Cell Transplantation
MH  - Histological Techniques
MH  - Immunohistochemistry
MH  - Leukocytes, Mononuclear/transplantation
MH  - Liver Cirrhosis, Experimental/chemically induced/*therapy
MH  - Liver Function Tests
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Polymerase Chain Reaction
EDAT- 2010/11/26 06:00
MHDA- 2011/12/13 00:00
CRDT- 2010/11/25 06:00
PHST- 2010/11/25 06:00 [entrez]
PHST- 2010/11/26 06:00 [pubmed]
PHST- 2011/12/13 00:00 [medline]
AID - 10.1111/j.1478-3231.2010.02364.x [doi]
PST - ppublish
SO  - Liver Int. 2011 Aug;31(7):932-9. doi: 10.1111/j.1478-3231.2010.02364.x. Epub 2010 
      Nov 24.