PMID- 21092675
OWN - NLM
STAT- MEDLINE
DCOM- 20111027
LR  - 20101124
IS  - 1673-0860 (Print)
IS  - 1673-0860 (Linking)
VI  - 45
IP  - 9
DP  - 2010 Sep
TI  - [Expression and clinical significance of CCR6, CCR7 and CD4(+)CD25(+) Foxp3(+) 
      regulatory T cells in laryngeal squamous cell carcinoma and neck lymphatic 
      metastasis].
PG  - 759-64
AB  - OBJECTIVE: To evaluate the expressions and clinical significance of chemokine 
      receptor 6 (CCR6), chemokine receptor 7(CCR7) and CD4(+)CD25(+)Foxp3(+) 
      regulatory T cells (Treg) in laryngeal squamous cell carcinoma (LSCC) and 
      metastatic lymph nodes. METHODS: Blood samples and fresh specimens of LSCC were 
      obtained from 50 LSCC patients treated. Blood samples from normal subjects were 
      donated by 20 volunteers. The mRNA expressions of CCR6, CCR7 and their ligands 
      CCL20, CCL19/CCL21 were detected by real-time quantitative RT-PCR. Expressions of 
      CCR6 and CCR7 proteins were examined with immunohistochemistry. Flow cytometry 
      was used to investigate Treg in peripheral blood mononuclear cells. RESULTS: The 
      relative expression levels of CCR6, CCR7 and CCL19 mRNA in tumor tissues with 
      lymphatic metastases were significantly lower than those without lymph node 
      metastases (P < 0.05), while the relative expression level of CCL20 mRNA in tumor 
      tissues with lymphatic metastases was significantly higher than that of those 
      without metastatic lymph nodes (t = 2.39, P < 0.05). Immunohistochemistry showed 
      that the expression levels of CCR6 and CCR7 in tumor tissues in patients with 
      lymph node metastases than those without lymph node metastases. CCR6 and CCR7 
      expressions were also detected in metastatic lymph nodes. Flow cytometry showed 
      that the percentage of Treg in blood in LSCC patients was significantly higher 
      than that in normal subjects (t = 2.19, P < 0.05), and those with lymph node 
      metastasis had a much higher percentage of Treg (t = 2.14, P < 0.05). 
      CONCLUSIONS: CCR6, CCR7 and Treg could take part in the process of lymph node 
      metastasis in LSCC patients.
FAU - Chen, Bin
AU  - Chen B
AD  - Department of Otolaryngology, Eye Ear Nose & Throat Hospital of Fudan University, 
      Shanghai 200031, China.
FAU - Lu, Li-ming
AU  - Lu LM
FAU - Tao, Lei
AU  - Tao L
FAU - Zhou, Liang
AU  - Zhou L
FAU - Li, Shi-min
AU  - Li SM
FAU - Zhu, Li
AU  - Zhu L
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhonghua Er Bi Yan Hou Tou Jing Wai Ke Za Zhi
JT  - Zhonghua er bi yan hou tou jing wai ke za zhi = Chinese journal of 
      otorhinolaryngology head and neck surgery
JID - 101247574
RN  - 0 (CCR6 protein, human)
RN  - 0 (CCR7 protein, human)
RN  - 0 (FOXP3 protein, human)
RN  - 0 (Forkhead Transcription Factors)
RN  - 0 (Interleukin-2 Receptor alpha Subunit)
RN  - 0 (Receptors, CCR6)
RN  - 0 (Receptors, CCR7)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Carcinoma, Squamous Cell/*immunology/metabolism/pathology
MH  - Female
MH  - Flow Cytometry
MH  - Forkhead Transcription Factors/metabolism
MH  - Humans
MH  - Interleukin-2 Receptor alpha Subunit/metabolism
MH  - Laryngeal Neoplasms/*immunology/metabolism/pathology
MH  - Lymphatic Metastasis
MH  - Male
MH  - Middle Aged
MH  - Receptors, CCR6/*metabolism
MH  - Receptors, CCR7/*metabolism
MH  - T-Lymphocytes, Regulatory/*immunology/metabolism
EDAT- 2010/11/26 06:00
MHDA- 2011/10/28 06:00
CRDT- 2010/11/25 06:00
PHST- 2010/11/25 06:00 [entrez]
PHST- 2010/11/26 06:00 [pubmed]
PHST- 2011/10/28 06:00 [medline]
PST - ppublish
SO  - Zhonghua Er Bi Yan Hou Tou Jing Wai Ke Za Zhi. 2010 Sep;45(9):759-64.