PMID- 21094153
OWN - NLM
STAT- MEDLINE
DCOM- 20110324
LR  - 20181201
IS  - 1873-3492 (Electronic)
IS  - 0009-8981 (Linking)
VI  - 412
IP  - 3-4
DP  - 2011 Jan 30
TI  - Studies on the effects of heparin products on pregnancy-associated plasma protein 
      A.
PG  - 376-81
LID - 10.1016/j.cca.2010.11.024 [doi]
AB  - BACKGROUND: Intravenous low molecular weight (LMWH) and unfractionated heparin 
      (UFH) increase the circulating concentrations of pregnancy-associated plasma 
      protein A (PAPP-A), a novel cardiac risk marker, in haemodialysis and coronary 
      angiography patients. METHODS: To further investigate the mechanisms of heparin 
      effects, free PAPP-A was analysed in serial serum samples collected during 
      haemodialysis (intravenous LMWH), carotid endarterectomy or abdominal aortic 
      aneurysm surgery (intravenous UFH), treatment at intensive care unit 
      (subcutaneous LMWH), and coronary angiography (intravenous bivalirudin). PAPP-A 
      was extracted from plaque tissue samples of endarterectomy and aneurysm patients. 
      The interaction between heparin products and free PAPP-A was studied with gel 
      filtration. RESULTS: After intravenous UFH and LMWH free PAPP-A increased 
      significantly but bivalirudin had no effect. After LMWH bolus in haemodialysis 
      patients 85% of free PAPP-A was cleared with a half-life of 13.1 min and the rest 
      with a half-life of 96.6 min. Subcutaneous LMWH led to lower and slower free 
      PAPP-A elevation. PAPP-A extracted from plaque tissues was in free form and 
      extraction was strongly enhanced by LMWH. Heparin products increased the 
      molecular size of free PAPP-A. CONCLUSIONS: The heparin-induced PAPP-A elevation 
      is seen in various patients and should be taken into account when PAPP-A is 
      studied as a biomarker.
CI  - Copyright (c) 2010 Elsevier B.V. All rights reserved.
FAU - Wittfooth, Saara
AU  - Wittfooth S
AD  - Department of Biotechnology, University of Turku, Turku, Finland. 
      saara.wittfooth@utu.fi
FAU - Tertti, Risto
AU  - Tertti R
FAU - Lepantalo, Mauri
AU  - Lepantalo M
FAU - Porela, Pekka
AU  - Porela P
FAU - Qin, Qiu-Ping
AU  - Qin QP
FAU - Tynjala, Joanna
AU  - Tynjala J
FAU - Inkinen, Outi
AU  - Inkinen O
FAU - Perttila, Juha
AU  - Perttila J
FAU - Airaksinen, K E Juhani
AU  - Airaksinen KE
FAU - Pettersson, Kim
AU  - Pettersson K
LA  - eng
PT  - Journal Article
DEP - 20101119
PL  - Netherlands
TA  - Clin Chim Acta
JT  - Clinica chimica acta; international journal of clinical chemistry
JID - 1302422
RN  - 0 (Anticoagulants)
RN  - 0 (Antithrombins)
RN  - 0 (Heparin, Low-Molecular-Weight)
RN  - 0 (Hirudins)
RN  - 0 (Peptide Fragments)
RN  - 0 (Recombinant Proteins)
RN  - EC 3.4.24.- (Pregnancy-Associated Plasma Protein-A)
RN  - TN9BEX005G (bivalirudin)
SB  - IM
MH  - Aged
MH  - Anticoagulants/administration & dosage/pharmacokinetics/*pharmacology
MH  - Antithrombins/pharmacology
MH  - Female
MH  - Heparin, Low-Molecular-Weight/administration & 
      dosage/pharmacokinetics/*pharmacology
MH  - Hirudins/pharmacology
MH  - Humans
MH  - Male
MH  - Molecular Weight
MH  - Peptide Fragments/pharmacology
MH  - Pregnancy
MH  - Pregnancy-Associated Plasma Protein-A/chemistry/*metabolism
MH  - Recombinant Proteins/pharmacology
MH  - Renal Dialysis
MH  - Vascular Diseases/blood/metabolism/pathology/surgery
MH  - Vascular Surgical Procedures
EDAT- 2010/11/26 06:00
MHDA- 2011/03/25 06:00
CRDT- 2010/11/25 06:00
PHST- 2010/08/12 00:00 [received]
PHST- 2010/10/21 00:00 [revised]
PHST- 2010/11/14 00:00 [accepted]
PHST- 2010/11/25 06:00 [entrez]
PHST- 2010/11/26 06:00 [pubmed]
PHST- 2011/03/25 06:00 [medline]
AID - S0009-8981(10)00706-0 [pii]
AID - 10.1016/j.cca.2010.11.024 [doi]
PST - ppublish
SO  - Clin Chim Acta. 2011 Jan 30;412(3-4):376-81. doi: 10.1016/j.cca.2010.11.024. Epub 
      2010 Nov 19.