PMID- 21095165
OWN - NLM
STAT- MEDLINE
DCOM- 20110404
LR  - 20131121
IS  - 1873-376X (Electronic)
IS  - 1570-0232 (Linking)
VI  - 878
IP  - 32
DP  - 2010 Dec 15
TI  - Determination of beta-lactam antibiotics in milk based on magnetic molecularly 
      imprinted polymer extraction coupled with liquid chromatography-tandem mass 
      spectrometry.
PG  - 3421-6
LID - 10.1016/j.jchromb.2010.10.030 [doi]
AB  - In this work, a rapid and selective method was successfully developed using the 
      magnetic molecularly imprinted polymer (MMIP) as sorbent for the extraction of 
      beta-lactam antibiotics (BLAs) from milk samples. The MMIP has been prepared using 
      penicillin V potassium (PENV) as template molecule, methacrylic acid as 
      functional monomer, ethylene glycol dimethacrylate as crosslinking agent and 
      Fe(3)O(4) magnetite as magnetic component. The experimental results showed that 
      the MMIP had high affinity and selectivity toward PENV and other structurally 
      related BLAs. The extraction process was carried out in a single step by mixing 
      the extraction solvent, MMIPs and milk samples under ultrasonic action. When the 
      extraction was completed, the MMIPs adsorbing the analytes were separated from 
      the sample matrix by an external magnet. The analytes eluted from the MMIP were 
      analyzed by liquid chromatography-tandem mass spectrometry. For achieving optimal 
      preconcentration and reducing non-specific interactions, various parameters 
      affecting the extraction efficiency such as extraction mode, extraction solvent, 
      the amount of MMIPs, extraction time, washing solution and eluting solution were 
      comprehensively evaluated. Under the optimal conditions, the detection limits of 
      BLAs are in the range of 1.6-2.8 ng mL(-1). The relative standard deviations of 
      intra- and inter-day ranging from 3.2% to 8.3% and from 3.6% to 9.8% are 
      obtained, respectively. The method was applied to determine BLAs including PENV, 
      amoxicillin and oxacillin in five milk samples from different provenances. The 
      recoveries of BLAs in these samples from 71.6% to 90.7% are obtained.
CI  - Copyright (c) 2010 Elsevier B.V. All rights reserved.
FAU - Zhang, Xiaopan
AU  - Zhang X
AD  - College of Chemistry, Jilin University, 2699 Qianjin Street, Changchun 130012, 
      China.
FAU - Chen, Ligang
AU  - Chen L
FAU - Xu, Yang
AU  - Xu Y
FAU - Wang, Hui
AU  - Wang H
FAU - Zeng, Qinglei
AU  - Zeng Q
FAU - Zhao, Qi
AU  - Zhao Q
FAU - Ren, Nanqi
AU  - Ren N
FAU - Ding, Lan
AU  - Ding L
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20101104
PL  - Netherlands
TA  - J Chromatogr B Analyt Technol Biomed Life Sci
JT  - Journal of chromatography. B, Analytical technologies in the biomedical and life 
      sciences
JID - 101139554
RN  - 0 (beta-Lactams)
RN  - Z61I075U2W (Penicillin V)
SB  - IM
MH  - Animals
MH  - Chemical Fractionation/methods
MH  - Chromatography, Liquid/*methods
MH  - Food Analysis/*methods
MH  - Linear Models
MH  - Magnetics
MH  - Milk/*chemistry
MH  - Molecular Imprinting
MH  - Penicillin V/chemistry
MH  - Reproducibility of Results
MH  - Sensitivity and Specificity
MH  - Tandem Mass Spectrometry/*methods
MH  - beta-Lactams/*analysis
EDAT- 2010/11/26 06:00
MHDA- 2011/04/05 06:00
CRDT- 2010/11/25 06:00
PHST- 2010/07/17 00:00 [received]
PHST- 2010/10/12 00:00 [revised]
PHST- 2010/10/27 00:00 [accepted]
PHST- 2010/11/25 06:00 [entrez]
PHST- 2010/11/26 06:00 [pubmed]
PHST- 2011/04/05 06:00 [medline]
AID - S1570-0232(10)00675-6 [pii]
AID - 10.1016/j.jchromb.2010.10.030 [doi]
PST - ppublish
SO  - J Chromatogr B Analyt Technol Biomed Life Sci. 2010 Dec 15;878(32):3421-6. doi: 
      10.1016/j.jchromb.2010.10.030. Epub 2010 Nov 4.