PMID- 21097715
OWN - NLM
STAT- MEDLINE
DCOM- 20111020
LR  - 20220311
IS  - 1544-2217 (Electronic)
IS  - 0300-9858 (Linking)
VI  - 48
IP  - 3
DP  - 2011 May
TI  - Histopathologic features, immunophenotyping, clonality, and eubacterial 
      fluorescence in situ hybridization in cats with lymphocytic 
      cholangitis/cholangiohepatitis.
PG  - 627-41
LID - 10.1177/0300985810384409 [doi]
AB  - Feline lymphocytic cholangitis is a poorly characterized disease complex with 
      respect to histologic lesions, immunophenotype, and etiopathogenesis. 
      Seventy-eight cases of feline lymphocytic cholangitis (n = 51) and feline hepatic 
      lymphoma (n = 27) were reviewed using standardized histopathology, 
      immunophenotyping (B cell and T cell), polymerase chain reaction for T-cell 
      receptor (TCR) gene rearrangement, and fluorescence in situ hybridization (FISH) 
      for eubacteria. Five histopathologic features in cases of lymphocytic cholangitis 
      assisted in its differentiation from hepatic lymphoma: bile duct targeting (n = 
      32, 62.7%), ductopenia (n = 9, 17.6%), peribiliary fibrosis (n = 37, 72.5%), 
      portal B-cell aggregates (n = 36, 70.6%), and portal lipogranulomas (n = 38, 
      74.5%). The majority of lymphocytic cholangitis cases (n = 35, 68.6%) were T cell 
      predominant; 15 (29.4%) had an equal mix of B cells and T cells, and 1 (1.9%) had 
      a B cell-predominant infiltrate; 66.6% of hepatic lymphoma cases were T-cell 
      lymphomas. TCR clonality results were unexpected, with 17.1% of cases of 
      lymphocytic cholangitis having clonal or oligoclonal populations and with T-cell 
      lymphomas having variable TCR clonality (63.6% clonal or oligoclonal, 36.3% 
      polyclonal). The majority of lymphocytic cholangitis (n = 32 of 36, 88.8%) and 
      all hepatic lymphoma cases had no detectable eubacteria using FISH. As 
      demonstrated here, bile duct targeting, ductopenia, peribiliary fibrosis, portal 
      B-cell aggregates, and portal lipogranulomas are lymphocytic cholangitis features 
      that, along with polyclonal TCR (83%), help differentiate it from hepatic 
      lymphoma. No strong evidence was found implicating in situ bacterial colonization 
      as an etiopathogenesis of lymphocytic cholangitis.
FAU - Warren, A
AU  - Warren A
AD  - Department of Veterinary Clinical and Diagnostic Sciences, University of Calgary, 
      Calgary, Canada. alwarren@ucalgary.ca
FAU - Center, S
AU  - Center S
FAU - McDonough, S
AU  - McDonough S
FAU - Chiotti, R
AU  - Chiotti R
FAU - Goldstein, R
AU  - Goldstein R
FAU - Meseck, E
AU  - Meseck E
FAU - Jacobsen, M
AU  - Jacobsen M
FAU - Rowland, P
AU  - Rowland P
FAU - Simpson, K
AU  - Simpson K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20101119
PL  - United States
TA  - Vet Pathol
JT  - Veterinary pathology
JID - 0312020
SB  - IM
MH  - Animals
MH  - Cat Diseases/genetics/microbiology/*pathology
MH  - Cats
MH  - Cholangitis/genetics/pathology/*veterinary
MH  - Female
MH  - Genetic Predisposition to Disease
MH  - Hepatitis, Animal/genetics/*pathology
MH  - Immunohistochemistry/veterinary
MH  - Immunophenotyping/*veterinary
MH  - Liver/pathology
MH  - Male
MH  - Polymerase Chain Reaction
MH  - Retrospective Studies
EDAT- 2010/11/26 06:00
MHDA- 2011/10/21 06:00
CRDT- 2010/11/25 06:00
PHST- 2010/11/25 06:00 [entrez]
PHST- 2010/11/26 06:00 [pubmed]
PHST- 2011/10/21 06:00 [medline]
AID - 0300985810384409 [pii]
AID - 10.1177/0300985810384409 [doi]
PST - ppublish
SO  - Vet Pathol. 2011 May;48(3):627-41. doi: 10.1177/0300985810384409. Epub 2010 Nov 
      19.