PMID- 21098015
OWN - NLM
STAT- MEDLINE
DCOM- 20110930
LR  - 20191224
IS  - 1460-2385 (Electronic)
IS  - 0931-0509 (Linking)
VI  - 26
IP  - 6
DP  - 2011 Jun
TI  - Specific collaboration between rat membrane complement regulators Crry and CD59 
      protects peritoneum from damage by autologous complement activation.
PG  - 1821-30
LID - 10.1093/ndt/gfq683 [doi]
AB  - BACKGROUND: The peritoneal cavity is isolated from the outside and is usually a 
      sterile environment. Patients on peritoneal dialysis (PD) have PD fluid (PDF) 
      infused into the peritoneal cavity. We previously showed that unregulated 
      complement activation could contribute to the development of peritoneal 
      inflammation in yeast peritonitis in PD therapy. In that situation, suppression 
      of local complement activation is essential to protect the host from further 
      injury. The membrane complement regulators (CRegs), Crry, CD55 and CD59, are 
      expressed in the rat peritoneum, especially along the mesothelial cell layer. 
      METHODS: We investigated CRegs' functional roles in the peritoneal cavity using 
      blocking mAb against each CReg and complement activation in different PDFs. 
      RESULTS: Blockade of any single CReg did not cause spontaneous peritoneal injury 
      in rat. Combined blockade of Crry and CD59 induced focal peritoneal tissue injury 
      and heavy accumulation of inflammatory cells with peritoneal edema at 24 h. 
      Deposits of C3 and C5b-9 were found on the peritoneal surface after combined 
      blocking of Crry and CD59. Systemic complement depletion by cobra venom factor 
      abrogated these inflammatory changes. When combined blockade of Crry and CD59 was 
      performed with PDF of different pH and glucose concentration in rats, the 
      peritoneal injuries were enhanced with lower pH and higher glucose concentration. 
      These results were confirmed by in vitro experiments using primary rat 
      mesothelial cell culture. CONCLUSIONS: Rat CRegs, Crry and CD59, specifically 
      collaborate to control complement activation in rat peritoneum. During PD, 
      impairment of CReg might contribute to the development of severe peritoneal 
      inflammation.
FAU - Mizuno, Tomohiro
AU  - Mizuno T
AD  - Neuropsychopharmacology and Hospital Pharmacy, Nagoya University Graduate School 
      of Medicine, Nagoya, Japan.
FAU - Mizuno, Masashi
AU  - Mizuno M
FAU - Morgan, B Paul
AU  - Morgan BP
FAU - Noda, Yukihiro
AU  - Noda Y
FAU - Yamada, Kiyofumi
AU  - Yamada K
FAU - Okada, Noriko
AU  - Okada N
FAU - Yuzawa, Yukio
AU  - Yuzawa Y
FAU - Matsuo, Seiichi
AU  - Matsuo S
FAU - Ito, Yasuhiko
AU  - Ito Y
LA  - eng
GR  - 068590/Wellcome Trust/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20101122
PL  - England
TA  - Nephrol Dial Transplant
JT  - Nephrology, dialysis, transplantation : official publication of the European 
      Dialysis and Transplant Association - European Renal Association
JID - 8706402
RN  - 0 (Antigens, Surface)
RN  - 0 (CD55 Antigens)
RN  - 0 (CD59 Antigens)
RN  - 0 (Complement C3)
RN  - 0 (Complement Membrane Attack Complex)
RN  - 0 (Cr1l protein, rat)
RN  - 0 (Receptors, Cell Surface)
SB  - IM
MH  - Animals
MH  - Antigens, Surface/*metabolism
MH  - CD55 Antigens/metabolism
MH  - CD59 Antigens/*metabolism
MH  - Cells, Cultured
MH  - *Complement Activation
MH  - Complement C3/*metabolism
MH  - Complement Membrane Attack Complex/*metabolism
MH  - Edema/*prevention & control
MH  - Epithelium
MH  - Immunoenzyme Techniques
MH  - Male
MH  - Peritoneal Dialysis
MH  - Peritoneum/injuries/*metabolism/pathology
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptors, Cell Surface/*metabolism
EDAT- 2010/11/26 06:00
MHDA- 2011/10/01 06:00
CRDT- 2010/11/25 06:00
PHST- 2010/11/25 06:00 [entrez]
PHST- 2010/11/26 06:00 [pubmed]
PHST- 2011/10/01 06:00 [medline]
AID - gfq683 [pii]
AID - 10.1093/ndt/gfq683 [doi]
PST - ppublish
SO  - Nephrol Dial Transplant. 2011 Jun;26(6):1821-30. doi: 10.1093/ndt/gfq683. Epub 
      2010 Nov 22.