PMID- 21098849
OWN - NLM
STAT- MEDLINE
DCOM- 20110408
LR  - 20220331
IS  - 1550-9613 (Electronic)
IS  - 0278-4297 (Linking)
VI  - 29
IP  - 12
DP  - 2010 Dec
TI  - Effects of heart rate and anesthetic timing on high-resolution echocardiographic 
      assessment under isoflurane anesthesia in mice.
PG  - 1771-8
AB  - OBJECTIVE: Anesthesia provides sedation and immobility, facilitating 
      echocardiography in mice, but it influences cardiovascular function and therefore 
      outcomes of measurement. This study aimed to determine the effect of the optimal 
      heart rate (HR) and anesthetic timing on echocardiographic reproducibility under 
      isoflurane anesthesia. METHODS: Male C57BL/6J mice underwent high-resolution 
      echocardiography with relative fixed HRs and anesthetic timing. The same 
      experiment was repeated once again after 1 week. RESULTS: Echocardiography was 
      highly reproducible in repeated measurements under low-HR (350-400 beats per 
      minute [bpm]) and high-HR (475-525 bpm) conditions except some M-mode parameters 
      under low-HR conditions. With similar anesthetic timing, mice with a high HR had 
      decreased preload indices and increased ejection phase and Doppler indices. 
      Inversely, when the HR was similar, the echocardiographic results of mice under 
      short anesthetic timing showed little difference from the ones under long 
      anesthetic timing. CONCLUSIONS: This study shows that echocardiographic 
      assessment is greatly reproducible under a high HR. The HR is more important than 
      anesthetic timing for echocardiographic evaluation in mice.
FAU - Wu, Jian
AU  - Wu J
AD  - Shanghai Institute of Cardiovascular Diseases, Zhongshan Hospital and Institutes 
      of Biomedical Sciences, Fudan University, Shanghai, China.
FAU - Bu, Liping
AU  - Bu L
FAU - Gong, Hui
AU  - Gong H
FAU - Jiang, Guoliang
AU  - Jiang G
FAU - Li, Lei
AU  - Li L
FAU - Ma, Hong
AU  - Ma H
FAU - Zhou, Ning
AU  - Zhou N
FAU - Lin, Li
AU  - Lin L
FAU - Chen, Zhidan
AU  - Chen Z
FAU - Ye, Yong
AU  - Ye Y
FAU - Niu, Yuhong
AU  - Niu Y
FAU - Sun, Aijun
AU  - Sun A
FAU - Ge, Junbo
AU  - Ge J
FAU - Zou, Yunzeng
AU  - Zou Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Ultrasound Med
JT  - Journal of ultrasound in medicine : official journal of the American Institute of 
      Ultrasound in Medicine
JID - 8211547
RN  - 0 (Anesthetics, Inhalation)
RN  - CYS9AKD70P (Isoflurane)
SB  - IM
MH  - Anesthetics, Inhalation/*pharmacology
MH  - Animals
MH  - Echocardiography, Doppler/*methods
MH  - Heart Rate/*drug effects
MH  - Isoflurane/*pharmacology
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Reproducibility of Results
EDAT- 2010/11/26 06:00
MHDA- 2011/04/09 06:00
CRDT- 2010/11/25 06:00
PHST- 2010/11/25 06:00 [entrez]
PHST- 2010/11/26 06:00 [pubmed]
PHST- 2011/04/09 06:00 [medline]
AID - 29/12/1771 [pii]
AID - 10.7863/jum.2010.29.12.1771 [doi]
PST - ppublish
SO  - J Ultrasound Med. 2010 Dec;29(12):1771-8. doi: 10.7863/jum.2010.29.12.1771.