PMID- 21104144
OWN - NLM
STAT- MEDLINE
DCOM- 20110726
LR  - 20220321
IS  - 1573-4978 (Electronic)
IS  - 0301-4851 (Linking)
VI  - 38
IP  - 4
DP  - 2011 Apr
TI  - Nucleotide variation and physical mapping of ribosomal genes using FISH in genus 
      Tor (Pisces, Cyprinidae).
PG  - 2637-47
LID - 10.1007/s11033-010-0405-7 [doi]
AB  - Molecular cytogenetic studies were carried out for localization of 18S and 5S 
      ribosomal DNAs on chromosomes of three cyprinid fish species viz., T. khudree, T. 
      mussullah and T. mosal mahanadicus using two color fluorescence in situ 
      hybridization (FISH). All the species typically possessed 100 diploid chromosomes 
      with minor variation in karyo-morphology. The 18S rDNA signals were observed on 
      two pair of chromosomes in T. khudree and T. mussullah, and three pairs in T. 
      mosal mahanadicus. The location of 18S signals also showed affinity to silver 
      nitrate and chromomycin A3 staining. Similarly, variation in localization of 5S 
      rDNA among the three species has been detected with the presence of FISH signals 
      on one pair of chromosome in T. khudree and T. mussullah, and on two pairs in T. 
      mosal mahanadicus. These molecular markers could be used as species specific 
      markers for taxonomic identification and can further add in understanding the 
      dynamics of genome organization and karyotypic evolution of these species. The 
      18S rDNA region was sequenced that generated 1811, 1810 and 1776 bp long 18S 
      sequence in T. khudree, T. mussullah and T. mosal mahanadicus, respectively. The 
      18S rDNA sequence showed 95-98% identity among the subject species. Similarly, 5S 
      sequencing generated 203 bp long fragments in these species with 100% identity in 
      coding and 9.63% variability in non-transcribed spacer regions. The nucleotide 
      sequence variations could be used for understanding the genetic diversity and 
      will add new informative characters in comparative genomics. These results, in 
      general, would enhance the value and interpretation of ecological assessment data 
      for conservation of Tor species.
FAU - Mani, Indra
AU  - Mani I
AD  - National Bureau of Fish Genetic Resources, Canal Ring Road, P.O. Dilkusha, 
      Lucknow, 226 002, U.P., India.
FAU - Kumar, Ravindra
AU  - Kumar R
FAU - Singh, Mamta
AU  - Singh M
FAU - Nagpure, N S
AU  - Nagpure NS
FAU - Kushwaha, B
AU  - Kushwaha B
FAU - Srivastava, P K
AU  - Srivastava PK
FAU - Rao, D S Krishna
AU  - Rao DS
FAU - Lakra, W S
AU  - Lakra WS
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20101123
PL  - Netherlands
TA  - Mol Biol Rep
JT  - Molecular biology reports
JID - 0403234
RN  - 0 (DNA Primers)
RN  - 0 (DNA, Ribosomal)
RN  - 0 (Fluorescent Dyes)
RN  - DVW027E7NL (Chromomycin A3)
SB  - IM
MH  - Animals
MH  - Chromomycin A3
MH  - Computational Biology
MH  - Cyprinidae/*genetics
MH  - DNA Primers/genetics
MH  - DNA, Ribosomal/*genetics
MH  - Fluorescent Dyes
MH  - *Genetic Variation
MH  - In Situ Hybridization, Fluorescence
MH  - Microscopy, Fluorescence
MH  - *Physical Chromosome Mapping
MH  - Sequence Analysis, DNA
MH  - Silver Staining
MH  - Species Specificity
EDAT- 2010/11/26 06:00
MHDA- 2011/07/27 06:00
CRDT- 2010/11/25 06:00
PHST- 2010/03/27 00:00 [received]
PHST- 2010/11/08 00:00 [accepted]
PHST- 2010/11/25 06:00 [entrez]
PHST- 2010/11/26 06:00 [pubmed]
PHST- 2011/07/27 06:00 [medline]
AID - 10.1007/s11033-010-0405-7 [doi]
PST - ppublish
SO  - Mol Biol Rep. 2011 Apr;38(4):2637-47. doi: 10.1007/s11033-010-0405-7. Epub 2010 
      Nov 23.